pH-responsive polymeric micelle based on PEG-poly(β-amino ester)/(amido amine) as intelligent vehicle for magnetic resonance imaging in detection of cerebral ischemic area
pH-responsive polymeric micelle based on PEG-poly(β-amino ester)/(amido amine) as intelligent vehicle for magnetic resonance imaging in detection of cerebral ischemic area
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DOI：
10.1016/j.jconrel.2010.09.012
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发表时间：
2011-10-10
影响因子：
10.8
通讯作者：
Lee, Doo Sung
中科院分区：
文献类型：
--
作者：
Gao, Guang Hui;Lee, Jae Won;Lee, Doo Sung
开发了一系列pH响应性聚合物胶束作为智能载体，用于递送氧化铁（Fe（3）O（4））纳米颗粒并对酸性刺激环境快速响应，用于磁共振成像（MRI）。聚合物胶束可以在生理pH下通过嵌段共聚物自组装，所述嵌段共聚物由亲水性甲氧基聚（乙二醇）（PEG）和pH响应性聚（β-氨基酯）/（酰胺基胺）嵌段组成。因此，Fe（3）O（4）纳米粒子可以很好地封装到聚合物胶束由于疏水相互作用，由PEG冠屏蔽！壳然而，在酸性环境中，在其主链上具有可电离的叔氨基的pH响应性组分可以被质子化以溶解并释放疏水性Fe（3）O（4）纳米颗粒。用动态光散射（DLS）、超导量子干涉仪（SQUID）和3.0T磁共振成像仪对聚合物胶束进行了表征。为了评估这种MRI探针作为pH触发剂的能力，我们利用了脑缺血的疾病大鼠模型，该模型由于其病理条件而产生酸性组织。我们发现Fe（3）O（4）纳米颗粒在脑缺血区域逐渐积累，表明pH触发的MRI探针可能对靶向酸性环境和病理组织的诊断成像有效。(C)2010 Elsevier B. V.保留所有权利。
A series of pH-responsive polymeric micelles is developed to act as intelligent carriers to deliver iron oxide (Fe(3)O(4)) nanoparticles and respond rapidly to an acidic stimuli environment for magnetic resonance imaging (MRI). The polymeric micelle can be self-assembled at physiological pH by a block copolymer, consisting of a hydrophilic methoxy poly(ethylene glycol) (PEG) and a pH-responsive poly(beta-amino ester)/(amido amine) block. Consequently, the Fe(3)O(4) nanoparticles can be well encapsulated into polymeric micelles due to the hydrophobic interaction, shielded by a PEG corona! shell. In an acidic environment, however, the pH-responsive component, which has ionizable tert-amino groups on its backbone, can become protonated to be soluble and release the hydrophobic Fe(3)O(4) nanoparticles. The Fe(3)O(4)-loaded polymeric micelle was measured by dynamic light scattering (DLS), superconducting quantum interference device (SQUID) and a 3.0 T MRI scanner. To assess the ability of this MRI probe as a pH-triggered agent, we utilize a disease rat model of cerebral ischemia that produces acidic tissue due to its pathologic condition. We found gradual accumulation of Fe(3)O(4) nanoparticles in the brain ischemic area, indicating that the pH-triggered MRI probe may be effective for targeting the acidic environment and diagnostic imaging of pathologic tissue. (C) 2010 Elsevier B.V. All rights reserved.