U2 AS WELL AS U1 SMALL NUCLEAR RIBONUCLEOPROTEINS ARE INVOLVED IN PRE-MESSENGER RNA SPLICING
U2 AS WELL AS U1 SMALL NUCLEAR RIBONUCLEOPROTEINS ARE INVOLVED IN PRE-MESSENGER RNA SPLICING
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DOI：
10.1016/0092-8674(85)90270-3
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
STEITZ, JA
中科院分区：
文献类型：
--
作者：
BLACK, DL;CHABOT, B;STEITZ, JA
两种不同的实验方法提供的证据表明，U2和U1 snRNP在前体mRNA剪接功能。当使用核糖核酸酶H和与U2 RNA互补的两种脱氧寡核苷酸中的任一种选择性降解核提取物中的U2 snRNP时，剪接活性被消除。将其中U2已被降解的提取物与其中U1已被降解的提取物混合可恢复活性。抗（U-2）RNP自身抗体的使用证明U2 snRNP在体外剪接期间与前体RNA缔合。在60分钟时，但不是在0分钟时，包括分支点序列的内含子片段被抗（U2）RNP免疫沉淀到反应中。在任何时候，U1 snRNP都与前体mRNA的5“剪接位点结合。考虑了U2 snRNP与U1 snRNP和剪接过程中与前体mRNA的可能相互作用。
Two different experimental approaches have provided evidence that both U2 and U1 snRNPs function in premRNA splicing. When the U2 snRNPs in a nuclear extract are selectively degraded using ribonuclease H and either of two deoxyoligonucleotides complementary to U2 RNA, splicing activity is abolished. Mixing an extract in which U2 has been degraded with one in which U1 has been degraded recovers activity. Use of anti(U-2)RNP autoantibodies demonstrates that U2 snRNPs associate with the precursor RNA during in vitro splicing. At 60 min, but not at 0 min, into the reaction intron fragments that include the branch-point sequence are immunoprecipitated by anti-(U2)RNP. At all times, U1 snRNPs bind the 5'' splice site of the premRNA. Possible interactions of the U2 snRNP with the U1 snRNP and with the pre-mRNA during splicing are considered.