Greener extraction process and enhanced in vivo bioavailability of bioactive components from Carthamus tinctorius L. by natural deep eutectic solvents
Greener extraction process and enhanced in vivo bioavailability of bioactive components from Carthamus tinctorius L. by natural deep eutectic solvents
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天然低共熔溶剂提取红花生物活性成分的绿色工艺并提高体内生物利用度
DOI：
10.1016/j.foodchem.2021.129090
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发表时间：
2021-01-29
期刊：
影响因子：
8.8
通讯作者：
Li, Chang
中科院分区：
文献类型：
--
作者：
Tong, Xin;Yang, Jiehong;Li, Chang
天然低共熔溶剂（NaDES）由于其物理和化学优势，是广泛应用于天然产物提取的传统溶剂的有前途的绿色替代品。在本研究中，通过超声辅助提取（UAE）从红花中筛选生物活性化合物，筛选了 22 种由食品级成分组成的 NaDES。然后利用 HPLC-MS 技术研究了提取物中羟基红花黄 A (HSYA) 和脱水红花黄 B (ASYB) 在 SD 大鼠中的口服生物利用度。结果表明，L-脯氨酸-乙酰胺（L-Pro-Am）是一种有效的溶剂，HSYA和ASYB的产率分别为32.83和8.80 mg/g。药代动力学研究表明，口服L-Pro-Am提取物后，HSYA和ASYB的血液水平显着高于水提取物。特别是，HSYA和ASYB的相对生物利用度（相对于水提取物）经计算为183.5%和429.8%。
Natural deep eutectic solvents (NaDESs) are promising green alternatives to conventional solvents widely applied in the extraction of natural products due to their physical and chemical superiorities. In present study, 22 NaDESs consisted from food grade ingredients were screened in ultrasonic assisted extraction (UAE) of bioactive compounds from safflower. The oral bioavailabilities of hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (ASYB) in the extracts were then investigated in SD rats with the help of HPLC-MS technique. The results revealed that L-proline-acetamide (L-Pro-Am) was an effective solvent with the yields of HSYA and ASYB at 32.83 and 8.80 mg/g. Pharmacokinetic studies revealed that the blood level of HSYA and ASYB were significantly higher after oral administration of L-Pro-Am extract than that of aqueous extract. Especially, the relative bioavailabilities (to aqueous extract) of HSYA and ASYB were calculated 183.5% and 429.8%.