Rapid up-regulation of P2Y messengers during granulocytic differentiation of HL-60 cells
Rapid up-regulation of P2Y messengers during granulocytic differentiation of HL-60 cells
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DOI：
10.1016/s0014-5793(00)01618-5
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发表时间：
2000-06-09
期刊：
影响因子：
3.5
通讯作者：
Boeynaems, JM
中科院分区：
文献类型：
--
作者：
Communi, D;Janssens, R;Boeynaems, JM
HL-60细胞是表达两种ATP受体的人早幼粒细胞：P2 Y（2）和P2 Y（11）亚型。我们的北方印迹实验表明，在用粒细胞分化剂如视黄酸、二甲亚砜、粒细胞集落刺激因子、二丁酰环AMP和ATP处理后，这些细胞中的P2 Y（2）和P2 Y（11）信使迅速上调，且与蛋白质合成无关。AR-C67085和腺苷5 '-O-（3-硫代三磷酸）是重组P2 Y（11）受体的两种有效激动剂，其增加HL-60细胞内cAMP浓度的作用比ATP本身更强。这些结果支持ATP对HL-60细胞分化的作用是通过P2 Y（11）受体介导的结论。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
HL-60 cells are human promyelocytic cells expressing two ATP receptors: the P2Y(2) and P2Y(11) subtypes. Our Northern blotting experiments have shown that P2Y(2) and P2Y(11) messengers were up-regulated in these cells, rapidly and independently of protein synthesis, following treatment with granulocytic differentiating agents such as retinoic acid, dimethylsulfoxide, granulocyte-colony stimulating factor, dibutyryl cyclic AMP and ATP. AR-C67085 and adenosine 5'-O-(3-thiotriphosphate), two potent agonists of the recombinant P2Y(11) receptor, increased intracellular cAMP concentration in HL-60 cells more potently than ATP itself. These observations support the conclusion that the effect of ATP on HL-60 cell differentiation is mediated by the P2Y(11) receptor. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.