Global Ablation of Mouse Rab11a Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion.
Global Ablation of Mouse Rab11a Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion.
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小鼠 Rab11a 的整体消融会损害早期胚胎发生和基质金属蛋白酶分泌。
DOI：
10.1074/jbc.m113.538223
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Nan Gao
中科院分区：
文献类型：
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作者：
Shiyan Yu;Ghassan Yehia;Juanfei Wang;Ewa Stypulkowski;Ryotaro Sakamori;Ping Jiang;Edith B Hernandez-Enriquez;Tracy S. Tran;Edward M. Bonder;Wei Guo;Nan Gao
Rab11a被认为是循环内体的一个重要调节成分，在内吞和外吞网络中起着连接作用。Rab11a在小鼠胚胎发育中的精确性尚不清楚。我们全面消融Rab11 a，并检查Rab11无胚泡和小鼠胚胎成纤维细胞的表型和分子结果。使用多种贩运试验和互补分析，我们确定，在多个重要的膜相关和可溶性货物，Rab11a囊泡交通多种可溶性MMP的分泌的关键贡献。Rab11的空胚能够正常形成囊胚，但在围着床期死亡。我们的数据表明，Rab11a关键控制小鼠囊胚发育和可溶性基质金属蛋白酶分泌。
Rab11a has been conceived as a prominent regulatory component of the recycling endosome, which acts as a nexus in the endo- and exocytotic networks. The precisein vivorole of Rab11a in mouse embryonic development is unknown. We globally ablated Rab11a and examined the phenotypic and molecular outcomes in Rab11anullblastocysts and mouse embryonic fibroblasts. Using multiple trafficking assays and complementation analyses, we determined, among multiple important membrane-associated and soluble cargos, the critical contribution of Rab11a vesicular traffic to the secretion of multiple soluble MMPs. Rab11anullembryos were able to properly form normal blastocysts but died at peri-implantation stages. Our data suggest that Rab11a critically controls mouse blastocyst development and soluble matrix metalloproteinase secretion.