HAPTEN-INDUCED MODEL OF CHRONIC INFLAMMATION AND ULCERATION IN THE RAT COLON
HAPTEN-INDUCED MODEL OF CHRONIC INFLAMMATION AND ULCERATION IN THE RAT COLON
复制标题
DOI：
10.1016/0016-5085(89)90904-9
复制
发表时间：
1989-03-01
期刊：
影响因子：
29.4
通讯作者：
WALLACE, JL
中科院分区：
文献类型：
--
作者：
MORRIS, GP;BECK, PL;WALLACE, JL
我们开发了一个简单的和可重复的大鼠慢性结肠炎症模型，通过在腔内灌注含有“屏障破坏者”和半抗原的溶液。半抗原2,4,6-三硝基苯磺酸（5- 30mg）在0.25 ml 50%的乙醇中作为“屏障破坏者”，会产生剂量依赖性的结肠溃疡和炎症。在30mg剂量下，三硝基苯磺酸/乙醇诱导的溃疡和明显的肠壁增厚持续至少8周。组织学上，炎症反应包括粘膜和粘膜下浸润，多形核白细胞、巨噬细胞、淋巴细胞、结缔组织肥大细胞和成纤维细胞。炎症诱导后3周死亡的大鼠中有57%出现肉芽肿。还观察到朗汉s型巨细胞。节段性溃疡和炎症是常见的。该模型引起的炎症和溃疡的特征和相对较长的持续时间为在特定控制的方式下研究结肠炎症性疾病的病理生理提供了机会，并评估可能适用于人类炎症性肠病的新治疗方法。
We have developed a simple and reproducible rat model of chronic colonic inflammation by the intraluminal instillation of a solution containing a "barrier breaker" and a hapten. Administration of the hapten 2,4,6-trinitrobenzenesulfonic acid (5-30 mg) in 0.25 ml of 50% ethanol as the "barrier breaker" produced dose-dependent colonic ulceration and inflammation. At a dose of 30 mg, trinitrobenzenesulfonic acid/ethanol-induced ulceration and marked thickening of the bowel wall persisted for at least 8 wk. Histologically, the inflammatory response included mucosal and submucosal infiltration by polymorphonuclear leukocytes, macrophages, lymphocytes, connective tissue mast cells, and fibroblasts. Granulomas were observed in 57% of the rats killed 3 wk after induction of inflammation. Langhan''s-type giant cells were also observed. Segmental ulceration and inflammation were common. The characteristics and relatively long duration of inflammation and ulceration induced in this model afford an opportunity to study the pathophysiology of colonic inflammatory disease in a specifically controlled fashion, and to evaluate new treatments potentially applicable to inflammatory bowel disease in humans.