GLOMERULAR LOCALIZATION OF PLATELET FACTOR-IV IN STREPTOCOCCAL NEPHRITIS
GLOMERULAR LOCALIZATION OF PLATELET FACTOR-IV IN STREPTOCOCCAL NEPHRITIS
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DOI：
10.1159/000186835
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发表时间：
1992-05-01
期刊：
影响因子：
2.5
通讯作者：
MEZZANO, D
中科院分区：
文献类型：
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作者：
MEZZANO, S;BURGOS, ME;MEZZANO, D
由于血小板第4因子(PF4)是一种阳离子(等电点为7.6)的血小板分泌蛋白，在体外和体内都能与肾小球多聚阴离子亲和，并参与中性粒细胞的趋化，因此我们用间接免疫荧光显微镜观察了链球菌性肾炎(APSGN)患者肾小球中是否有PF4沉积。对照组用非免疫性山羊血清或用人PF4预吸附的抗PF4血清作为一抗。在所研究的20名患者中，有14名患者的肾组织中发现了PF4的肾小球沉积，其中9名患者的肾小球沉积特别强烈。PF4与系膜和毛细血管壁结合。肾小球内PF4沉积与尿蛋白水平呈显著正相关(p=0.024)。这些发现为血小板在APSGN发病机制中的作用提供了进一步的证据，并提示PF4可能有助于改变APSGN的肾小球通透性。
Since platelet factor 4 (PF4), a cationic (pI 7.6) platelet secretory protein, binds avidly to glomerular polyanions both in vitro and in vivo, and is implicated in neutrophil chemotaxis, we studied by indirect immunofluorescence microscopy the presence of PF4 deposits in glomeruli of patients with poststreptococcal nephritis (APSGN).Goat antihuman PF4 serum was used as primary antibody and fluorescein-conjugated IgG fraction of rabbit antigoat IgG as second antibody. Controls consisted of nonimmune goat serum or anti-PF4 serum preabsorbed with human PF4, as primary antibodies. Glomerular deposits of PF4 were demonstrated in renal tissues obtained by biopsy in 14 of 20 patients studied; the deposits were particularly intense in 9 patients. PF4 was bound to the mesangium and to the capillary walls. There was a significant positive correlation between intraglomerular deposits of PF4 and the levels of proteinuria (p = 0.024). These findings provide further evidence for a role of platelets in the pathogenesis of APSGN and suggest that PF4 may contribute to alter the glomerular permeability in this disease.