Determination of junction avidity of cytolytic T cell and target cell.
Determination of junction avidity of cytolytic T cell and target cell.
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DOI：
10.1126/science.3491426
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发表时间：
1986-12
期刊：
影响因子：
56.9
通讯作者：
K. Sung;L. Sung;M. Crimmins;S. Burakoff;S. Chien
中科院分区：
文献类型：
--
作者：
K. Sung;L. Sung;M. Crimmins;S. Burakoff;S. Chien
通过使用生物物理方法实现了细胞毒性T淋巴细胞与其靶细胞之间的连接的亲合力的直接测量。使用显微操作技术来确定在递送致死命中之前将细胞毒性T细胞（人克隆F1，具有HLA-DRw 6特异性）与其特异性靶细胞（JY：HLA-A2，-B7，-DR 4，w 6）分离所需的力。分离F1-JY对所需的力为每平方厘米1.5 × 10（4）达因。F1-JY对的这种连接亲合力是F1-F1和JY-JY对的6至13倍; F1-JY缀合物需要更强的分离力，并且比同源细胞对更容易重新连接。这项研究提供了一个估计的亲和力的细胞毒性T细胞为他们的靶细胞和洞察的生物物理相关的分子复合物中形成的相互作用的细胞毒性T细胞和他们的目标在细胞毒性过程中。
A direct measurement of the avidity of the junction between a cytotoxic T lymphocyte and its target cell was achieved by using a biophysical approach. A micromanipulation technique was used to determine the force required to separate a cytotoxic T cell (human clone F1, with specificity for HLA-DRw6) from its specific target cell (JY: HLA-A2, -B7, -DR4, w6) prior to delivery of the lethal hit. The force required to separate the F1-JY pair is 1.5 X 10(4) dynes per square centimeter. This junction avidity for F1-JY pairs is 6 to 13 times greater than that for F1-F1 and JY-JY pairs; the F1-JY conjugate requires a stronger separating force and is more easily rejoined than the homologous cell pairs. This study provides an estimate of the avidity of cytotoxic T cells for their target cells and insights into the biophysical correlates of the molecular complexes formed in the interaction of cytotoxic T cells and their targets during the cytotoxic process.