Pseudomonas humi sp. nov., isolated from leaf soil
Pseudomonas humi sp. nov., isolated from leaf soil
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DOI：
10.1007/s00203-018-1588-x
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发表时间：
2019-03-01
影响因子：
2.8
通讯作者：
Hoshino, Tamotsu
中科院分区：
文献类型：
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作者：
Akita, Hironaga;Kimura, Zen-ichiro;Hoshino, Tamotsu
从日本采集的叶土中分离到一株好氧、革兰氏阴性、不产芽孢、能动、杆状和木质素降解的细菌菌株，假单胞菌CCA 1。该菌株在20-45 ℃（最适20 ℃）、pH 5.0-10.0（最适pH 5.0）和2%NaCl存在下生长。其主要细胞脂肪酸为C-16：0和总特征8（C（18：1）6c和/或C（18：1）7 c）。主要的醌系统是泛醌-9。比较16 S rRNA基因序列分析表明，假单胞菌属CCA 1与香茅假单胞菌NBRC 103043（T）最接近（98.9%），但基于atpD、gyrA、gyrB和rpoB基因序列片段的多位点序列分析表明，菌株CCA 1与其最接近的假单胞菌型菌株分开为分支。CCA 1与近缘种的模式菌株之间的DNA-DNA杂交值小于53%。基于其表型、化学分类学和系统发育特征，我们提出假单胞菌属中的一个新种，并将其命名为Humi假单胞菌新种。模式菌株为CCA 1（=HUT 8136（T）=TBRC 8616（T））。
An aerobic, Gram-negative, non-sporulating, motile, rod-shaped and lignin-degrading bacterial strain, Pseudomonas sp. CCA1, was isolated from leaf soil collected in Japan. This strain grew at 20-45 degrees C (optimum 20 degrees C), at pH 5.0-10.0 (optimum pH 5.0), and in the presence of 2% NaCl. Its major cellular fatty acids were C-16:0 and summed feature 8 (C(18:1)6c and/or C(18:1)7c). The predominant quinone system was ubiquinone-9. Comparative 16S rRNA gene sequence analysis showed that Pseudomonas sp. CCA1 was related most closely to P. citronellolis NBRC 103043(T) (98.9%), but multilocus sequence analysis based on fragments of the atpD, gyrA, gyrB and rpoB gene sequences showed strain CCA1 to branch separately from its most closely related Pseudomonas type strains. DNA-DNA hybridization values between Pseudomonas sp. CCA1 and type strains of closely related Pseudomonas species were less than 53%. Based on its phenotypic, chemotaxonomic and phylogenetic features, we propose that Pseudomonas sp. CCA1 represents a novel species within the genus Pseudomonas, for which the name Pseudomonas humi sp. nov. is proposed. The type strain is CCA1 (=HUT 8136(T)=TBRC 8616(T)).