Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production
Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production
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DOI：
10.1007/978-1-4939-2205-5_2
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发表时间：
2015-01-01
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影响因子：
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通讯作者：
Oppezzo, Pablo
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文献类型：
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作者：
Correa, Agustin;Oppezzo, Pablo
尽管重组蛋白的生产在学术界和工业领域具有重要意义，但许多关于可溶性和均相产物表达的问题仍未得到解决。虽然制定了若干战略来克服这些障碍，但目前还没有适用于所有情况的灵丹妙药。实际上，需要对每种蛋白的几个关键表达参数进行评估。在不同的表达蛋白的宿主中，大肠杆菌是目前使用最广泛的。在本章中，我们回顾了许多用来规避蛋白质不溶性问题的不同工具。
Despite the importance of recombinant protein production in academy and industrial fields, many issues concerning the expression of soluble and homogeneous product are still unsolved. Although several strategies were developed to overcome these obstacles, at present there is no magic bullet that can be applied for all cases. Indeed, several key expression parameters need to be evaluated for each protein. Among the different hosts for protein expression, Escherichia coli is by far the most widely used. In this chapter, we review many of the different tools employed to circumvent protein insolubility problems.