Mesenchymal stem cells derived from peripheral blood protects against ischemia
Mesenchymal stem cells derived from peripheral blood protects against ischemia
复制标题
DOI：
10.1089/neu.2006.0161
复制
发表时间：
2007-03-01
影响因子：
4.2
通讯作者：
Kocsis, Jeffery D.
中科院分区：
文献类型：
--
作者：
Ukai, Ryo;Honmou, Osamu;Kocsis, Jeffery D.
在大鼠脑缺血模型中，静脉输注骨髓制备的间充质干细胞（MSCs）可减少梗死体积并改善功能缺陷。骨髓间质干细胞样多能前体细胞（PMSCs）也被认为存在于外周血中。为了验证骨髓间充质干细胞治疗可能对中风有治疗益处的假设，我们比较了骨髓间充质干细胞和骨髓间充质干细胞全身输送的疗效。用微丝封堵脑腔内血管诱导大鼠永久性大脑中动脉闭塞。制备大鼠骨髓间充质干细胞和PMSCs， MCAO后6 h静脉注射。在第6小时、第1、3和7天通过MR成像和组织学评估病变大小。同时采用灌注加权图像（PWI）评价脑卒中时脑血流动力学的变化。使用跑步机压力测试评估功能结局。与对照组相比，骨髓间充质干细胞和PMSCs治疗组均减少了病变体积，改善了局部脑血流，并改善了功能。两组间质干细胞治疗的疗效相似。这些数据表明，来自外周血的PMSCs可能是中风细胞治疗的重要细胞来源。
Intravenous delivery of mesenchymal stem cells (MSCs) prepared from bone marrow (BMSCs) reduces infarction volume and ameliorates functional deficits in a rat cerebral ischemia model. MSC-like multipotent precursor cells (PMSCs) have also been suggested to exist in peripheral blood. To test the hypothesis that treatment with PMSCs may have a therapeutic benefit in stroke, we compared the efficacy of systemic delivery of BMSCs and PMSCs. A permanent middle cerebral artery occlusion (MCAO) in rat was induced by intraluminal vascular occlusion with a microfilament. Rat BMSCs and PMSCs were prepared in culture and intravenously injected into the rats 6 h after MCAO. Lesion size was assessed at 6 h, and 1, 3, and 7 days using MR imaging and histology. The hemodynamic change of cerebral blood perfusion on stroke was assessed the same times using perfusion-weighted image (PWI). Functional outcome was assessed using the treadmill stress test. Both BMSCs and PMSCs treated groups had reduced lesion volume, improved regional cerebral blood flow, and functional improvement compared to the control group. The therapeutic benefits of both MSC-treated groups were similar. These data suggest that PMSCs derived from peripheral blood could be an important cell source of cell therapy for stroke.