The mitochondrial DNA of Dictyostelium discoideum:: complete sequence, gene content and genome organization
The mitochondrial DNA of Dictyostelium discoideum:: complete sequence, gene content and genome organization
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DOI：
10.1007/pl00008685
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发表时间：
2000-04-01
期刊：
影响因子：
--
通讯作者：
Tanaka, Y
中科院分区：
文献类型：
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作者：
Ogawa, S;Yoshino, R;Tanaka, Y
我们提出了一个概述的基因内容和组织的线粒体基因组的盘基网柄藻。线粒体基因组由55，564 bp组成，A + T含量为72.6%。已鉴定的基因包括两种核糖体RNA的基因（III和rns），18个tRNA，10个IADH脱氢酶复合物亚基（nad 1、2、3、4、4L、5、6、7、9和11）、脱辅基细胞色素B（cytB）、细胞色素氧化酶的三个亚基（cox 1/2和3）、ATP合酶复合物的四个亚基（atp 1、6、8和9）、15种核糖体蛋白和5种其它ORF（不包括内含子ORF）。D. discoideum mtDNA包括以下内容。(1)所有基因都编码在同一条DNA链上，并使用通用遗传密码。(2)cox 1基因没有终止密码子，与下游cox 2基因融合。13个核糖体蛋白基因和4个ORF基因组成一个长约15.4kb的基因簇，其中有几个基因重叠。(3)基因组中编码的tRNA数量不足以支持线粒体蛋白质的合成。(4)总共有五个I组内含子位于rnl和cox 1/2中，其中cox 1/2中的三个包含四个独立的ORF。我们将基因组与其他已测序的线粒体基因组进行比较，特别是卡氏阿米巴。
We present an overview of the gene content and organization of the mitochondrial genome of Dictyostelium discoideum. The mitochondria genome consists of 55,564 bp with an A + T content of 72.6%. The identified genes include those for two ribosomal RNAs (I ill and rns), 18 tRNAs, ten subunits of the lADH dehydrogenase complex (nad1, 2, 3, 4, 4L, 5, 6, 7, 9 and 11), apocytochrome b (cytb), three subunits of the cytochrome oxidase (cox1/2 and 3), four subunits of the ATP synthase complex (atp1, 6, 8 and 9), 15 ribosomal proteins, and five other ORFs, excluding intronic ORFs. Notable features of D. discoideum mtDNA include the following. (1) All genes are encoded on the same strand of the DNA and a universal genetic code is used. (2) The cox1 gene has no termination codon and is fused to the downstream cox2 gene. The 13 genes for ribosomal proteins and four ORF genes form a cluster 15.4 kb long with several gene overlaps. (3) The number of tRNAs encoded in the genome is not sufficient to support the synthesis of mitochondrial protein. (4) In total, five group I introns reside in rnl and cox1/2, and three of those in cox1/2 contain four free-standing ORFs. We compare the genome to other sequenced mitochondrial genomes, particularly that of Acanthamoeba castellanii.