14-3-3 proteins and plant development
14-3-3 proteins and plant development
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DOI：
10.1023/a:1021295604109
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发表时间：
2002-12-01
影响因子：
5.1
通讯作者：
Testerink, C
中科院分区：
文献类型：
--
作者：
Fulgosi, H;Soll, J;Testerink, C
14-3-3蛋白是一个普遍存在的调节分子家族，几乎在每一个真核生物和组织中都有发现。14-3-3蛋白于34年前被发现，首次在哺乳动物神经组织中被研究，但在过去的十年中，它们在各种植物调节和代谢途径中不可或缺的作用已被越来越多地确立。我们现在知道14-3-3成员调节氮同化和碳同化的基本过程，在淀粉合成、ATP产生、过氧化物解毒的调节中起辅助作用，并参与调节其他几个重要的生化途径。植物发育和种子萌发似乎也受14-3-3分子相互作用对其激活至关重要的因素的控制。14-3-3异构体位于细胞核内，是转录因子复合物的组成部分，并与脱落酸（ABA）诱导的基因表达机制的组成部分相互作用。此外，在动物细胞中，它们参与核-细胞质运输和分子隔离。细胞质14-33成员与叶绿体目标前蛋白形成一个引导复合体，并促进它们进入这些光合细胞器。近年来，已经在叶绿体中发现了几个14-3-3，它们可能参与类囊体蛋白的靶向和插入。14-3-3异构体特异性的鉴定，特别是14-3-3成员与生理反应之间的信号转导机制的阐明，是当前该领域研究的核心和发展主题。
The 14-3-3 proteins are a family of ubiquitous regulatory molecules which have been found in virtually every eukaryotic organism and tissue. Discovered 34 years ago, 14-3-3 proteins have first been studied in mammalian nervous tissues, but in the past decade their indispensable role in various plant regulatory and metabolic pathways has been increasingly established. We now know that 14-3-3 members regulate fundamental processes of nitrogen assimilation and carbon assimilation, play an auxiliary role in regulation of starch synthesis, ATP production, peroxide detoxification, and participate in modulation of several other important biochemical pathways. Plant development and seed germination appear also to be under control of factors whose interaction with 14-3-3 molecules is crucial for their activation. Located within the nucleus, 14-3-3 isoforms are constituents of transcription factor complexes and interact with components of abscisic acid (ABA)-induced gene expression machinery. In addition, in animal cells they participate in nucleo-cytoplasmic trafficking and molecular sequestration. Cytoplasmic 14-33 members form a guidance complex with chloroplast destined preproteins and facilitate their import into these photosynthetic organelles. Recently, several 14-3-3s have been identified within chloroplasts where they could be involved in targeting and insertion of thylakoid proteins. The identification of 14-3-3 isoform specificity, and in particular the elucidation of the signal transduction mechanisms connecting 14-3-3 members with physiological responses, are central and developing topics of current research in this field.