Partitioning of vasoactive intestinal polypeptide into lipid bilayers.
Partitioning of vasoactive intestinal polypeptide into lipid bilayers.
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将血管活性肠多肽分配成脂质双层。
DOI：
10.1016/0005-2736(94)90183-x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Paul,S
中科院分区：
文献类型：
--
作者：
Noda,Y;Rodriguez-Sierra,J;Liu,J;Landers,D;Mori,A;Paul,S
孵育的放射性标记的血管活性肠多肽（VIP）与预先形成的脂质囊泡组成的磷脂酰胆碱，磷脂酰甘油和胆固醇导致可逆和饱和协会的肽与脂质双层。反应的最适pH值在生理范围内。囊泡相关肽显示增强的稳定性蛋白水解消化，它被有效地释放到上清液中的洗涤剂溶解的囊泡，但仍然囊泡相关的治疗过程中与破坏离子相互作用的试剂。电中性囊泡的肽结合低于负性囊泡。电子自旋共振研究与5-doxylstearic酸或16-doxylstearic酸标记的囊泡表明，VIP降低的流动性接近的表面的双层和增加的流动性在其疏水核心。这些观察结果表明，VIP可以结合和渗透脂质双层。
Incubation of radiolabeled vasoactive intestinal polypeptide (VIP) with preformed lipid vesicles composed of phosphatidylcholine, phosphatidylglycerol and cholesterol resulted in reversible and saturable association of the peptide with the lipid bilayer. The pH-optimum for the reaction was in the physiological range. The vesicle-associated peptide displayed enhanced stability to proteolytic digestion, it was efficiently released into the supernatant by detergent-solubilization of the vesicles but remained vesicle-associated during treatment with agents that disrupt ionic interactions. Peptide binding by electrically neutral vesicles was lower than that by negative vesicles. Electron spin resonance studies with 5-doxylstearic acid or 16-doxylstearic acid labeled vesicles suggested that VIP decreased the fluidity close to the surface of the bilayer and increased the fluidity in its hydrophobic core. These observations suggest that VIP can bind and penetrate lipid bilayers.