Protection against pulmonary oxygen toxicity in rats by the intratracheal administration of liposome-encapsulated superoxide dismutase or catalase.
Protection against pulmonary oxygen toxicity in rats by the intratracheal administration of liposome-encapsulated superoxide dismutase or catalase.
复制标题
通过气管内施用脂质体封装的超氧化物歧化酶或过氧化氢酶来防止大鼠的肺氧中毒。
DOI：
10.1164/arrd.1985.132.1.164
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Hoidal,JR
中科院分区：
文献类型：
--
作者：
Padmanabhan,RV;Gudapaty,R;Liener,IE;Schwartz,BA;Hoidal,JR
为了测试使用脂质体将治疗剂递送至肺的可行性，在大鼠中研究了脂质体包封的超氧化物歧化酶（SOD）或过氧化氢酶对肺氧毒性的影响。将SOD或过氧化氢酶封装在带负电荷的多层脂质体中，并直接注入成年大鼠的气管，随后将其暴露于高氧（> 95%O2）。通过研究肺SOD和过氧化氢酶活性、存活率和肺形态学来检查对高氧的反应。接受脂质体包裹的SOD或过氧化氢酶的大鼠在高氧暴露24至72小时后，与对照组相比，肺匀浆中的酶活性水平增加。脂质体包封的SOD或过氧化氢酶处理的大鼠肺中的酶水平升高，伴随着高氧暴露72小时后的存活率显着改善，肺损伤比其他对照组少。
To test the feasibility of using liposomes to deliver therapeutic agents to the lungs, the effect of liposome-encapsulated superoxide dismutase (SOD) or catalase on pulmonary oxygen toxicity was studied in rats. The SOD or catalase was encapsulated in negatively charged multilamellar liposomes and administered directly into the trachea of adult rats, which were subsequently exposed to hyperoxia (> 95% O2). Response to hyperoxia was examined by studying lung SOD and catalase activities, survival rates, and lung morphology. Rats receiving liposome-encapsulated SOD or catalase showed increased levels of enzyme activities in the lung homogenates compared with those in the control groups after 24 to 72 h of hyperoxic exposure. Elevated enzyme levels in the lungs of rats treated with liposome-encapsulated SOD or catalase were accompanied by a significant improvement in survival rates after 72 h of hyperoxic exposure and less lung injury than in the other control groups.