Eosinophils and mast cells in bronchoalveolar lavage in subjects with mild asthma. Relationship to bronchial hyperreactivity.
Eosinophils and mast cells in bronchoalveolar lavage in subjects with mild asthma. Relationship to bronchial hyperreactivity.
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DOI：
10.1164/ajrccm/137.1.62
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
A. Wardlaw;S. Dunnette;G. Gleich;J. Collins;A. Kay
中科院分区：
文献类型：
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作者：
A. Wardlaw;S. Dunnette;G. Gleich;J. Collins;A. Kay
我们对17例轻度特应性哮喘患者（9例有症状，8例无症状）和14例非哮喘对照者（6例花粉热，8例非特应性）进行了支气管肺泡灌洗（BAL）。有一个显着的增加，肥大细胞的百分比在这两组哮喘患者，虽然计数是没有什么不同，从以前报道的一些其他呼吸系统疾病。气道高反应性哮喘患者（PC 20小于4 mg/ml）的自发组胺释放显著增加。有症状的哮喘患者支气管肺泡灌洗液中嗜酸性粒细胞计数和主要碱性蛋白（MBP）浓度显著升高。此外，MBP回收量与BAL中嗜酸性粒细胞百分比之间存在显著相关性。当将气道高反应性的哮喘患者与气道反应性正常的受试者进行比较时，这些变化更加明显。此外，在高反应性哮喘患者中，上皮细胞的百分比显著增加。PC 20与BAL中肥大细胞（p <0.01）、嗜酸性粒细胞（p <0.05）和上皮细胞（p <0.05）的百分比以及MBP量（p <0.01）之间呈负相关。这项研究支持了这一假设，即支气管高反应性是继发于上皮细胞损伤介导的嗜酸性粒细胞衍生的颗粒产品。
We have performed bronchoalveolar lavage (BAL) on 17 subjects with mild atopic asthma (9 symptomatic, 8 asymptomatic) and 14 nonasthmatic control subjects (6 hay fever, 8 nonatopic). There was a significant increase in the percentage of mast cells in both groups of asthmatics although the counts were no different from those previously reported for a number of other respiratory diseases. Asthmatics with airway hyperreactivity (PC20 less than 4 mg/ml) had significant increases in spontaneous histamine release. There was a significant elevation in the eosinophil count and the concentration of major basic protein (MBP) in BAL fluid in the symptomatic asthmatics. Furthermore, there was a significant correlation between the amounts of MBP recovered and the percentage of eosinophils in the BAL. These changes were even more marked when asthmatics with airway hyperreactivity were compared with subjects with normoreactive airways. In addition, there was a significant increase in the percentage of epithelial cells in the hyperreactive asthmatics. There was an inverse correlation between the PC20 and the percentage of mast cells (p less than 0.01), eosinophils (p less than 0.05), and epithelial cells (p less than 0.05) and amount of MBP in BAL (p less than 0.01). This study supports the hypothesis that bronchial hyperresponsiveness is secondary to epithelial cell damage mediated through eosinophil-derived granule products.