Engineering Controllable Pulse Generation for Molecular Communication via Genetic Circuits
Engineering Controllable Pulse Generation for Molecular Communication via Genetic Circuits
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DOI：
10.1109/tmbmc.2023.3252942
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
Dadi Bi;Yansha Deng
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文献类型：
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作者：
Dadi Bi;Yansha Deng
合成生物学为工程界提供了一种工具，可以构建能够执行所需信号处理功能的生物实体。表现出自然行为的遗传电路的设计和研究可以有助于更好地理解基因表达行为背后的原理和动力学，以及用于合成生物学的工程细胞系统。在本文中，我们提出了一个合成生物系统能够产生脉冲形状的信号，这是一个普遍的行为，在自然环境中。特别是，我们提出的脉冲产生系统将脉冲产生功能划分到不同的细胞中，这些细胞通过细胞间信号分子连接。更具体地说，该系统由三个具有不同数字逻辑功能的工程单元组成，并且脉冲生成利用逻辑状态的改变。为了定量描述产生的脉冲，我们不仅使用Shea-Ackers形式主义分析每个细胞的个体行为，而且推导出细胞间的信号通道。仿真结果表明，脉冲形状的信号可以产生在一个可控的方式，通过安排在不同的空间配置的细胞。
Synthetic biology provides the engineering community with a tool to build biological entities that are capable of carrying out desired signal processing functionalities. The design and study of genetic circuits that exhibit natural behavior can be helpful for an improved understanding of the principles and kinetics behind the gene expression behavior, as well as for engineering cellular systems for synthetic biology. In this paper, we propose a synthetic biology system capable of generating a pulse-shaped signal, which is a prevalent behavior in natural environment. In particular, our proposed pulse generation system divides the pulse generation functions into different cells which are connected by intercellular signaling molecules. More specifically, the system is consisted of three engineered cells with different digital logic functionalities, and the pulse generation exploits the alteration in logic state. To quantitatively describe the generated pulse, we not only analyze the individual behavior of each cell using Shea-Ackers formalism but also derive the intercellular signaling channel. Simulation results demonstrate that the pulse-shaped signal can be produced in a controllable manner by arranging cells in different spatial configurations.