Trypanosomiasis in Venezuelan water buffaloes:: association of packed-cell volumes with seroprevalence and current trypanosome infection
Trypanosomiasis in Venezuelan water buffaloes:: association of packed-cell volumes with seroprevalence and current trypanosome infection
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DOI：
10.1179/136485906x91521
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发表时间：
2006-06-01
影响因子：
--
通讯作者：
Mendoza-Leon, A.
中科院分区：
文献类型：
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作者：
Garcia, H.;Garcia, M. -E.;Mendoza-Leon, A.
采用IFAT和微红细胞压差离心技术分别对委内瑞拉最重要畜牧地区的水牛中锥虫病的血清流行率和当前锥虫感染的流行率进行了评估。还评估了以pcr为基础的测定法鉴定水牛中锥虫种类的有效性。在644只动物中，40只（6.2%）被血液离心检测出锥虫感染，196只（30.4%）被IFAT检测出抗锥虫抗体阳性。基于聚合酶链反应的检测结果表明，目前感染的动物中有92.5%感染了间日锥虫，其余感染了氏滴虫（首次在委内瑞拉水牛中证实了氏滴虫）。治疗和预防水牛中锥虫感染的国家规划似乎没有取得巨大成功，尽管它的重点是间日疟原虫。尽管在检测到的感染中，有28只（70%）的寄生虫血症水平被归类为低水平（并且在感染动物中，堆积细胞体积似乎与IFAT结果无关，也与寄生虫血症水平无关），但40只感染水牛的平均堆积细胞体积显著低于未感染动物（P < 0.05）。因此，应使农民认识到由锥虫引起的水牛生产力损失的可能性。
The seroprevalence of trypanosomiasis and the prevalence of current trypanosome infection in water buffaloes from the most important livestock areas of Venezuela were evaluated by IFAT and the microhaematocrit centrifugation technique, respectively. The usefulness of a PCR-based assay for identifying the trypanosome species in the buffaloes was also evaluated. Of the 644 animals investigated, 40 (6.2%) were found infected with trypanosomes by blood centrifugation, and 196 (30.4%) were found positive for anti-trypanosome antibodies, by IFAT. The results of the PCR-based assay indicated that 92.5% of the animals with current infections were infected with Trypanosoma vivax and the rest with T. theileri (the first molecular confirmation of T. theileri in Venezuelan water buffaloes). The national programme to treat and prevent trypanosome infections in the buffaloes does not appear to be meeting with great success, even though it is focused on T. vivax. Although the level of parasitaemia was categorized as low for 28 (70%) of the infections detected (and packed-cell volumes appeared to be unassociated with IFAT result, and uncorrelated, in the infected animals, with level of parasitaemia), the 40 infected buffaloes had a significantly lower mean packed-cell volume than the uninfected animals (P < 0.05). Farmers should therefore be made aware of the probability of trypanosome-attributable losses in buffalo productivity.