Differently Expressed Circular RNAs in Lacrimal Sacs From Patients With Chronic Dacryocystitis.
Differently Expressed Circular RNAs in Lacrimal Sacs From Patients With Chronic Dacryocystitis.
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DOI：
10.3389/fgene.2022.834111
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发表时间：
2022
影响因子：
3.7
通讯作者：
Xiao C
中科院分区：
文献类型：
--
作者：
Li Y;Liu X;Zhang W;Song X;Zhang L;Xiao C
本研究旨在鉴定慢性泪囊炎患者泪囊中不同表达的环状RNA（circRNA），并探讨其潜在作用。收集3例慢性泪囊炎患者和3例对照者的泪囊样本，在核糖体RNA耗尽后进行RNA测序。将共同表达的circRNA和信使RNA（mRNA）用于共表达分析。利用miRanda软件构建了CircRNA-microRNA（miRNA）-mRNA相互作用网络。同时对circRNA-mRNA共表达网络中下调和上调的mRNA进行了途径和功能富集分析。通过定量实时逆转录聚合酶链反应（qRT-PCR）验证慢性泪囊炎和对照样本中circRNA和mRNA的表达水平。在通过RNA测序预测的所有3，909个circRNA中，25个circRNA（20个上调和5个下调）在慢性泪囊炎样本中表达不同。此外，还鉴定了1，486个差异表达的mRNA。在这些不同表达的circRNA和mRNA中，8种通过qRT-PCR验证，包括MYH2、DSP、CD27、CCL5、FN 1、has_circ_0004792、has_circ_0001062和has_circ_0115476。基因本体论（GO）分析表明，在该共表达网络中，大多数改变的mRNA参与免疫系统过程，同时京都基因和基因组百科全书（KEGG）分析显示，这些改变表达的mRNA也在上皮细胞的细菌侵袭中扩增，这两者被认为参与慢性泪囊炎的发病机制。在circRNA-miRNA-mRNA相互作用网络中，发现6个circRNA与Th1和Th2细胞分化相关，而Th1和Th2细胞分化与慢性泪囊炎的发生发展密切相关。本研究确定了慢性泪囊炎患者和对照个体泪囊样本的circRNA和mRNA之间的统计学显著差异，并提供了对circRNA、miRNA和mRNA在慢性泪囊炎发病机制中的调节机制的新见解。
This study was designed to identify differently expressed circular RNAs (circRNAs) and investigate their potential roles in lacrimal sacs from patients with chronic dacryocystitis. The lacrimal sac samples of three chronic dacryocystitis patients and three control subjects were collected for RNA sequencing after ribosomal RNA was depleted. Differently expressed circRNAs and messenger RNAs (mRNAs) were used for co-expression analysis. CircRNA-microRNA (miRNA)-mRNA interaction network were also established by miRanda software. Meanwhile, pathway and functional enrichment analysis were conducted for the down- and up-regulated mRNAs in the circRNA-mRNA co-expression network. The expression levels of circRNAs and mRNAs in chronic dacryocystitis and control samples were validated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). In all the 3,909 circRNAs predicted through RNA sequencing, 25 circRNAs (20 up-regulated and 5 down-regulated) expressed differently in chronic dacryocystitis samples. Besides, there identified 1,486 differentially expressed mRNAs. Of these differently expressed circRNAs and mRNAs, eight were validated by qRT-PCR, including MYH2, DSP, CD27, CCL5, FN1, has_circ_0004792, has_circ_0001062, and has_circ_0115476. Gene Ontology (GO) analysis indicated that the majority of altered mRNAs in this co-expression network were involved in immune system processes and meanwhile Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these altered expressed mRNAs were also amplified in bacterial invasion of epithelial cells, both of which were thought to be involved in the pathogenesis of chronic dacryocystitis. In the circRNA-miRNA-mRNA interaction network, six circRNAs were found to be related to Th1 and Th2 cell differentiation, which was closely associated with the development of chronic dacryocystitis. This study identified statistically significant differences between circRNAs and mRNAs of lacrimal sac samples of chronic dacryocystitis patients and control individuals and provides novel insight into the regulatory mechanism of circRNAs, miRNAs, and mRNAs in the pathogenesis of chronic dacryocystitis.
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