THE LOCALIZED REQUIREMENTS FOR A GENE AFFECTING SEGMENTATION IN DROSOPHILA - ANALYSIS OF LARVAE MOSAIC FOR RUNT
THE LOCALIZED REQUIREMENTS FOR A GENE AFFECTING SEGMENTATION IN DROSOPHILA - ANALYSIS OF LARVAE MOSAIC FOR RUNT
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DOI：
10.1016/0012-1606(85)90459-2
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
WIESCHAUS, EF
中科院分区：
文献类型：
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作者：
GERGEN, JP;WIESCHAUS, EF
在发育中的果蝇胚胎中需要有侏儒基因来进行适当的分割。突变胚胎不能孵化，但会分泌出一种形态缺陷明显的幼虫角质层。在小胚胎中，沿着动物的前后轴，每隔2节段出现模式缺失。删除的区域将被剩余区域的镜像副本替换。本研究通过分析幼虫遗传嵌合体的分割模式，探讨了对runt+活性的局部需求。这种分析是由模糊和剃须婴儿突变，影响幼虫角质层形态而不影响分割辅助。这2个突变作为幼虫角质层由基因小细胞分泌区域的标记。对小块拼接模式的分析表明，该基因对片段的影响主要是细胞自主的。这包括模式删除和相关的镜像重复。这表明镜像复制不是由于再生，而是由于对胚胎模式的更直接的影响。马赛克图案还揭示了幼虫表皮图案形成过程的其他方面。在此基础上，提出了一种幼虫模式的生成模型。
The runt gene is required in a developing Drosophila embryo for proper segmentation. Mutant embryos fail to hatch but secrete a larval cuticle in which pattern defects are apparent. In runt embryos, there are pattern deletions spaced at 2 segment intervals along the antero-posterior axis of the animal. The deleted regions are replaced by mirror-image duplications of the remaining regions. This study investigates the localized requirements for runt+ activity by analyzing the segmentation patterns in larval genetic mosaics. This analysis is aided by the faintoid and shavenbaby mutations which affect larval cuticle morphology without affecting segmentation. These 2 mutations serve as markers of the regions of larval cuticle secreted by genetically runt cells. The analysis of the runt mosaic patterns indicates the effects of the gene on segmentation are primarily cell autonomous. This includes both the pattern deletions and the associated mirror-image duplications. This indicates the mirror-image duplications are not due to regeneration but result from a more direct effect of runt on patterning in the embryo. The mosaic patterns also reveal other aspects of the process of pattern formation in the larval epidermis. Based on these results a model is presented for the generation of the larval pattern.