PHENOTYPICALLY DISTINCT SUBSETS OF CD4(+) T-CELLS INDUCE OR PROTECT FROM CHRONIC INTESTINAL INFLAMMATION IN C - B-17 SCID MICE
PHENOTYPICALLY DISTINCT SUBSETS OF CD4(+) T-CELLS INDUCE OR PROTECT FROM CHRONIC INTESTINAL INFLAMMATION IN C - B-17 SCID MICE
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DOI：
10.1093/intimm/5.11.1461
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发表时间：
1993-11-01
影响因子：
4.4
通讯作者：
COFFMAN, RL
中科院分区：
文献类型：
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作者：
POWRIE, F;LEACH, MW;COFFMAN, RL
根据CD 45 RB决定子的表达水平，小鼠中的CD 4 + T细胞可细分为两个部分。以前的研究表明，这些子集在功能上是不同的。我们通过将这些亚群注射到C. B-17 scid小鼠。用CD 45 RB（高）CD 4 + T细胞群恢复的动物发展为致命的消耗性疾病，具有严重的单核细胞浸润到结肠中和IFN-γ mRNA水平升高。相反，用CD 45 RB（低）亚群或未分级的CD 4 + T细胞恢复的动物没有发生消耗性或结肠炎。重要的是，CD 45 RB（低）群体与CD 45 RB（高）群体的共转移预防了消耗性疾病和结肠炎。这些数据表明，重要的调节相互作用之间发生的CD 45 RB（高）和CD 45 RB（低）的CD 4 + T细胞亚群，这种机制的破坏具有致命的后果。
CD4+ T cells in the mouse can be subdivided into two fractions based on the level of expression of the CD45RB determinant. Previous studies have shown that these subsets are functionally distinct. We have further characterized the properties of these subpopulations in vivo by injecting them into C. B-17 scid mice. The animals restored with the CD45RB(high)CD4+ T cell population developed a lethal wasting disease with severe mononuclear cell infiltrates into the colon and elevated levels of IFN-gamma mRNA. In contrast, animals restored with the reciprocal CD45RB(low) subset or with unfractionated CD4+ T cells did not develop the wasting or colitis. Importantly, the co-transfer of the CD45RB(low) population with the CD45RB(high) population prevented the wasting disease and colitis. These data indicate that important regulatory interactions occur between the CD45RB(high) and CD45RB(low)CD4+ T cell subsets and that disruption of this mechanism has fatal consequences.