Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: a multiplex real-time PCR assay.
Simultaneous quantification of mitochondrial DNA copy number and deletion ratio: a multiplex real-time PCR assay.
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DOI：
10.1038/srep03887
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发表时间：
2014-01-27
影响因子：
4.6
通讯作者：
Roby RK
中科院分区：
文献类型：
--
作者：
Phillips NR;Sprouse ML;Roby RK
线粒体功能障碍与许多疾病和状况有关，如阿尔茨海默病、癌症和衰老。线粒体DNA （mtDNA）的改变可能为启动或传播这种功能障碍的过程提供见解。在这里，我们描述了一个独特的多重分析，同时提供评估mtDNA拷贝数和基因组的比例与共同的大缺失通过靶向两个线粒体位点和一个核位点。这种基于探针的单管多重检测提供了高特异性，同时消除了因单独分析核和线粒体目标而导致的井与井之间的差异。
Mitochondrial dysfunction is implicated in a vast array of diseases and conditions, such as Alzheimer's disease, cancer, and aging. Alterations in mitochondrial DNA (mtDNA) may provide insight into the processes that either initiate or propagate this dysfunction. Here, we describe a unique multiplex assay which simultaneously provides assessments of mtDNA copy number and the proportion of genomes with common large deletions by targeting two mitochondrial sites and one nuclear locus. This probe-based, single-tube multiplex provides high specificity while eliminating well-to-well variability that results from assaying nuclear and mitochondrial targets individually.