Bacterial status in root-filled teeth exposed to the oral environment by loss of restoration and fracture or caries - a histobacteriological study of treated cases
Bacterial status in root-filled teeth exposed to the oral environment by loss of restoration and fracture or caries - a histobacteriological study of treated cases
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DOI：
10.1046/j.1365-2591.2003.00721.x
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发表时间：
2003-11-01
影响因子：
5
通讯作者：
Bergenholtz, G
中科院分区：
文献类型：
--
作者：
Ricucci, D;Bergenholtz, G
目的描述牙齿的组织学和微生物的发现，根填充物已暴露于龋齿和口腔环境的一个延长的period.MethodologyFor列入研究，只有牙齿的随访期为3年或以上，那些一直没有适当的修复至少3个月的时间被认为是。一些牙根填充物已经好几年没有修复了。在所有，39根代表32颗牙齿进行了组织学检查。在5个牙根处观察到溶骨性病变。用改良的Brown/Brenn染色技术染色的纵向组织切片显示，在根管入口和牙本质小管中存在大量的细菌，但在除两个标本外的所有标本中均不存在根中部和根尖。软组织连接到根尖和根尖分支显示明显的炎性细胞浸润，表明微生物暴露在7的39根检查。在所有其他标本中，炎症细胞浸润要么不存在或稀疏，然后与挤出的根管material.Conclusions充分准备和填充根管抵抗细菌渗透，即使在坦率和长期的口腔暴露龋齿，骨折或损失的恢复。
Aim To describe histological and microbiological findings in teeth where root fillings had been exposed to caries and the oral environment for a prolonged period.MethodologyFor inclusion in the study, only teeth with a follow-up period of 3 years or more and those that had been without proper restoration for at least a period of 3 months were considered. Some root fillings had been without restoration for several years. In all, 39 roots representing 32 teeth were examined by histology.Results The majority of the specimens were without a discernible periapical bone lesion as assessed by radiography. Osteolytic lesions were seen with five roots. Longitudinal tissue sections stained with a modified Brown/Brenn staining technique revealed presence of stainable bacteria in abundance at the canal entrance and in dentinal tubules but were absent mid-root and apically in all but two specimens. Soft tissue attached to the root tip and in apical ramifications displayed distinct inflammatory cell infiltrates, suggesting microbial exposure in 7 of the 39 roots examined. In all other specimens, inflammatory cell infiltrates were either nonexistent or sparse and then associated with extruded sealer material.Conclusions Well-prepared and filled root canals resist bacterial penetration even upon frank and long-standing oral exposure by caries, fracture or loss of restoration.