Developmental links between the fetal and adult zones of the adrenal cortex revealed by lineage tracing.
Developmental links between the fetal and adult zones of the adrenal cortex revealed by lineage tracing.
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通过谱系追踪揭示胎儿和成人肾上腺皮质区域之间的发育联系。
DOI：
10.1128/mcb.00900-08
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发表时间：
2008
影响因子：
5.3
通讯作者：
Morohashi,Ken-ichirou
中科院分区：
文献类型：
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作者：
Zubair,Mohamad;Parker,KeithL;Morohashi,Ken-ichirou
核受体Ad4BP/SF-1对肾上腺皮质和性腺的发育至关重要，它们来源于一个共同的肾上腺性腺原基。肾上腺皮质随后形成形态上不同的区室：内区（胎儿区）和外区（终区或成体区）。尽管付出了相当大的努力，介导肾上腺和性腺原基以及胎儿和成人肾上腺皮质发育差异的机制仍然不完全清楚。我们之前在Ad4BP/SF-1基因座中发现了一个胎儿肾上腺特异性增强子（FAdE），该增强子引导转基因表达到胎儿肾上腺皮质，并证明该增强子受Ad4BP/SF-1的自动调节。我们现在将FAdE与Cre/loxPsystem结合起来，以追踪在发育的某个阶段中FAdE活跃的细胞谱系。这些谱系追踪研究明确地证实，成人皮层来源于胎儿皮层的前体细胞，在胚胎皮层中，在组织成两个不同的区域之前，FAdE被激活。这些胎儿肾上腺皮质细胞进入成人皮质细胞最终通路的潜力在胚胎第14.5天消失。因此，这些研究证明了胎儿和成人大脑皮层之间的直接联系，包括在特定发育阶段之前必须发生的过渡。
The nuclear receptor Ad4BP/SF-1 is essential for development of the adrenal cortex and the gonads, which derive from a common adrenogonadal primordium. The adrenal cortex subsequently forms morphologically distinct compartments: the inner (fetal) and outer (definitive or adult) zones. Despite considerable effort, the mechanisms that mediate the differential development of the adrenal and gonadal primordia and the fetal and adult adrenal cortices remain incompletely understood. We previously identified a fetal adrenal-specific enhancer (FAdE) in theAd4BP/SF-1locus that directs transgene expression to the fetal adrenal cortex and demonstrated that this enhancer is autoregulated by Ad4BP/SF-1. We now combine the FAdE with the Cre/loxPsystem to trace cell lineages in which the FAdE was active at some stage in development. These lineage-tracing studies establish definitively that the adult cortex derives from precursor cells in the fetal cortex in which the FAdE was activated before the organization into two distinct zones. The potential of these fetal adrenocortical cells to enter the pathway that eventuates in cells of the adult cortex disappeared by embryonic day 14.5. Thus, these studies demonstrate a direct link between the fetal and adult cortices involving a transition that must occur before a specific stage of development.