AXON INITIATION BY CILIARY NEURONS IN CULTURE
AXON INITIATION BY CILIARY NEURONS IN CULTURE
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DOI：
10.1016/0012-1606(78)90178-1
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发表时间：
1978-01-01
影响因子：
2.7
通讯作者：
COLLINS, F
中科院分区：
文献类型：
--
作者：
COLLINS, F
描述了一种用于研究轴突起始的神经培养系统。一个单独的小鸡胚胎纤毛神经元群体，不与其他细胞接触，附着在一个多聚氨基酸包被的培养皿上，暴露在心脏细胞条件培养基（HCM）中。在加入HCM后30分钟内，大多数神经元形成生长锥，到90分钟时，超过80%的神经元至少有1个轴突长于15.m。在加入HCM之前，睫状神经元在圆形细胞体的整个外围产生膜褶并延伸丝状足。添加HCM后，细胞表面发生了两种不同的变化：随机分布的表面运动组织成局部的高活性生长锥，然后形成轴突；以及细胞周围其他地方表面运动的停止。心脏细胞条件培养基可以通过增加神经细胞表面和基底之间的粘附性来诱导这些变化。
A nerve culture system for the study of axon initiation is described. A population of individual chick embryo ciliary neurons, free from contact with other cells and attached to a polyornithine-coated culture dish, is exposed to heart cell-conditioned medium (HCM). Within 30 min after the addition of HCM the majority of neurons have formed growth cones and by 90 min more than 80% of the neurons bear at least 1 axon longer than 15 .mu.m. Before the addition of HCM, ciliary neurons generate membrane ruffles and extend filopodia around the entire periphery of the rounded cell body. Axon initiation, following addition of HCM, consists of 2 distinctive changes in the cell surface: organization of the randomly distributed surface movements into localized highly active growth cones, which then form axons; and the cessation of surface movements elsewhere on the cell periphery. Heart cell-conditioned medium may induce these changes by increasing the adhesion between parts of the nerve cell surface and the substratum.