ONE-DIMENSIONAL AND 2-DIMENSIONAL NMR CHARACTERIZATION OF OXIDIZED AND REDUCED CYTOCHROME-C' FROM RHODOCYCLUS-GELATINOSUS
ONE-DIMENSIONAL AND 2-DIMENSIONAL NMR CHARACTERIZATION OF OXIDIZED AND REDUCED CYTOCHROME-C' FROM RHODOCYCLUS-GELATINOSUS
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DOI：
10.1021/bi00054a006
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发表时间：
1993-01-26
期刊：
影响因子：
2.9
通讯作者：
VILA, AJ
中科院分区：
文献类型：
--
作者：
BERTINI, I;GORI, G;VILA, AJ
记录了细胞色素c'Rhodocyclus gelatinosus的还原和氧化形式的1D和2D NMR谱。对铁形式的H-1核磁共振谱的pH依赖性进行了分析，并确定了两个主要的电离基团。通过比较细胞色素c′的可用光谱的pH依赖性，解决了先前研究中有关细胞色素c′的模糊性。利用二维谱，提出了铁和铁两种形式的所有顺磁位移信号的分配。
1D and 2D NMR spectra of both the reduced and oxidized forms of cytochrome c'Rhodocyclus gelatinosus have been recorded. The an lysis of the pH dependence of the H-1 NMR spectrum of the ferric form has been performed, and two main ionizing groups have been identified. By comparison of the pH dependence of the available spectra of cytochromes c', an ambiguity remaining from previous studies on related cytochromes c' has been solved. By means of 2D spectra, an assignment of all the paramagnetically shifted signals is proposed both for the ferrous and for the ferric forms.