MODIFICATION OF PHOSPHOLIPID MEMBRANE-STRUCTURE BY THE PLANT TOXIC PEPTIDE PYRULARIA THIONIN
MODIFICATION OF PHOSPHOLIPID MEMBRANE-STRUCTURE BY THE PLANT TOXIC PEPTIDE PYRULARIA THIONIN
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DOI：
10.1006/abbi.1993.1157
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发表时间：
1993-03-01
影响因子：
3.9
通讯作者：
ARIPOV, TF
中科院分区：
文献类型：
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作者：
GASANOV, SE;VERNON, LP;ARIPOV, TF
梨硫素又称P硫素，是一种从寄生植物中提取的生物活性多肽。通过测定不同磷脂组成时的磷光猝灭、EPR自旋标记、1H和31P核磁共振的响应，研究了其与磷脂膜相互作用的结构特征。在含有心磷脂或磷脂酰肌醇的磷脂酰胆碱双层膜中，P硫素可引起膜粘度显著增加，且在较高浓度时可观察到非双层结构的形成。在含有磷脂酰丝氨酸的磷脂酰胆碱双层膜中，P-硫素引起膜片层结构的显著改变，同时膜粘度降低。在所研究的所有脂类体系中，添加P硫素可引起膜通透性的增加，并诱导超声脂质体的融合。讨论了含磷脂酰丝氨酸的磷脂酰丝氨酸膜与P硫素相互作用的特殊性，提出了P硫素修饰磷脂膜的模型。
Pyrularia thionin (P thionin) is a bioactive peptide from the parasitic plantPyrularia pubera. The structural aspects of its interaction with phospholipid membranes were investigated by measuring the responses of phosphorescence quenching, EPR spin labels, and1H and31P NMR at different phospholipid compositions. In phosphatidylcholine bilayers containing cardiolipin or phosphatidylinositol, P thionin induced a pronounced increase in the membrane viscosity, and at higher P thionin concentrations the formation of nonbilayer structures was observed. In phosphatidylcholine bilayers containing phosphatidylserine, P thionin induced a significant transformation of membrane lamellar structure accompanied by a decrease of membrane viscosity. In all investigated lipid systems added P thionin caused an increase in membrane permeability and induced a fusion of sonicated liposomes. The specificity of P thionin interaction with phosphatidylserine-containing membrane is discussed and a model of phospholipid membrane modification by P thionin is suggested.