A COMPARISON OF METHODS FOR CALCULATING DNA DOUBLE-STRAND BREAK INDUCTION FREQUENCY IN MAMMALIAN-CELLS BY PULSED-FIELD GEL-ELECTROPHORESIS
A COMPARISON OF METHODS FOR CALCULATING DNA DOUBLE-STRAND BREAK INDUCTION FREQUENCY IN MAMMALIAN-CELLS BY PULSED-FIELD GEL-ELECTROPHORESIS
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DOI：
10.1080/09553009414550751
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发表时间：
1994-06-01
影响因子：
2.6
通讯作者：
MCMILLAN, TJ
中科院分区：
文献类型：
--
作者：
DEALMODOVAR, JMR;STEEL, GG;MCMILLAN, TJ
脉冲场电泳（PFGE）已成为评价辐射诱导的DNA双链断裂（dsb）的最广泛使用的方法之一。在大多数研究中，DNA从凝胶中的孔迁移的简单定量已被用作与dsb形成的相关性。在这里，我们比较了这样的方法，校准与I-125标记的UdR，与两种方法，其中涉及的DNA片段的大小分布的分析迁移在凝胶中。我们的结论是，三种方法产生的dsb诱导频率的绝对值相似。目前尚不清楚哪种方法是唯一的选择，但分析的比较增加了可以从PFGE实验中获得的信息。
Pulsed-field electrophoresis (PFGE) has become one of the most widely used methods for the evaluation of radiation-induced DNA double-strand breaks (dsb). In most studies a simple quantification of DNA migration from the well in the gel has been used as the correlate with dsb formation. Here we have compared such a method, as calibrated with I-125-labelled UdR, with two methods which involved the analysis of the distribution of sizes of DNA fragments migrating in the gel. We conclude that the three methods produce similar absolute values for dsb induction frequency. It is not clear which is the single method of choice but the comparision of the analyses increases the information which can be derived from PFGE experiments.