The Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme.
The Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme.
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Snf2 同源物 Fun30 充当同型二聚体 ATP 依赖性染色质重塑酶。
DOI：
10.1074/jbc.m109.082149
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发表时间：
2010-03-26
期刊：
影响因子：
--
通讯作者：
Hassan AH
中科院分区：
文献类型：
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作者：
Awad S;Ryan D;Prochasson P;Owen-Hughes T;Hassan AH
酿酒酵母Fun30（功能未知30）蛋白与Snf2相关ATP酶的扩展家族共享同源性。在这里，我们报告的纯化Fun30主要作为一个同源二聚体的分子量约为250 kDa。该复合物的生化特性表明，它具有ATP酶活性的DNA和染色质的刺激。与此一致，它也结合DNA和染色质。Fun30复合物在ATP依赖性染色质重塑测定中也表现出活性。有趣的是，它在组蛋白二聚体交换中的活性相对于重新定位核小体的能力是高的。Fun30还具有弱保守的CUE基序，这表明它可能与泛素化蛋白质特异性相互作用。然而，在体外Fun30被发现在其与泛素化组蛋白的相互作用没有特异性。
The Saccharomyces cerevisiae Fun30 (Function unknown now 30) protein shares homology with an extended family of Snf2-related ATPases. Here we report the purification of Fun30 principally as a homodimer with a molecular mass of about 250 kDa. Biochemical characterization of this complex reveals that it has ATPase activity stimulated by both DNA and chromatin. Consistent with this, it also binds to both DNA and chromatin. The Fun30 complex also exhibits activity in ATP-dependent chromatin remodeling assays. Interestingly, its activity in histone dimer exchange is high relative to the ability to reposition nucleosomes. Fun30 also possesses a weakly conserved CUE motif suggesting that it may interact specifically with ubiquitinylated proteins. However, in vitro Fun30 was found to have no specificity in its interaction with ubiquitinylated histones.