Glycoforms of CA125 antigen as a possible cancer marker
Glycoforms of CA125 antigen as a possible cancer marker
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DOI：
10.3233/cbm-2008-4104
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Milutinovic, Bojana S.
中科院分区：
文献类型：
--
作者：
Jankovic, Miroslava M.;Milutinovic, Bojana S.
CA 125是一种体腔上皮相关抗原，在正常和病理状态下均有表达。在这项研究中，我们比较了来自羊水和卵巢癌细胞系OVCAR-3的CA 125抗原的糖基化，以检测可能的差异作为其来源的特异性标记。将两种来源的抗原进行放射性标记，随后进行亲和层析，使用碳水化合物特异性不同的植物凝集素作为配体。将共同的色谱方案应用于所有柱，即它们用以下洗脱：a）洗涤缓冲液以洗出未结合的和低亲和力结合的级分，B）抑制性糖的溶液和c）低pH缓冲液，以释放高亲和力结合的级分。发现来自每个来源的CA 125抗原在存在多种糖型方面是异质的，其中O-连接的聚糖链占主导地位。然而，N-和O-连接的聚糖反应性凝集素的结合模式表明从羊水中分离的CA 125抗原和OVCAR-3细胞系之间的碳水化合物组成的明显差异。观察到的特异性CA 125-寡糖链可能是特别重要的生物医学方面，在其可能用于临床评价妇科功能的健康和疾病。
CA125, a coelomic epithelium-related antigen, is expressed in both normal and pathological conditions. In this study, we compared the glycosylation of CA125 antigen from amniotic fluid and the ovarian carcinoma cell line OVCAR-3, in order to detect possible differences as a specific marker of their origin. Antigens from both sources were radiolabelled and subsequently subjected to the affinity chromatography, using plant lectins differing in carbohydrate specificity as ligands. A common chromatographic scheme was applied to all columns, i.e. they were eluted with: a) washing buffer to wash out non-bound and low-affinity bound fractions, b) a solution of inhibitory sugar and c) a low pH buffer, to release the high affinity bound fractions. CA125 antigen from each source was found to be heterogeneous in respect to the existence of multiple glycoforms, with O-linked glycan chains predominating. However, the binding patterns of both N- and O-linked glycan-reactive lectins indicated distinct differences in carbohydrate composition between CA125 antigen isolated from amniotic fluid and OVCAR-3 cell line. The observed specificites of CA125-oligosaccharide chains might be of special importance from the biomedical aspect, in terms of their possible use for clinical evaluation of gynecological functions in health and disease.