GENETIC-STRUCTURE OF THE ENTEROCOCCUS-FAECALIS PLASMID PAD1-ENCODED CYTOLYTIC TOXIN SYSTEM AND ITS RELATIONSHIP TO LANTIBIOTIC DETERMINANTS
GENETIC-STRUCTURE OF THE ENTEROCOCCUS-FAECALIS PLASMID PAD1-ENCODED CYTOLYTIC TOXIN SYSTEM AND ITS RELATIONSHIP TO LANTIBIOTIC DETERMINANTS
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DOI：
10.1128/jb.176.23.7335-7344.1994
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发表时间：
1994-12-01
影响因子：
3.2
通讯作者：
CLEWELL, DB
中科院分区：
文献类型：
--
作者：
GILMORE, MS;SEGARRA, RA;CLEWELL, DB
信息素反应性共轭质粒是粪肠球菌所特有的。许多信息素响应质粒，包括那些经常从感染部位分离的质粒，表达一种新的细胞溶血素，具有溶血和细菌素活性。此外，这种细胞溶解素在几种疾病模型中已被证明是一种毒素。本研究采用核苷酸序列测定、诱变和互补分析等方法确定了粪肠杆菌质粒pAD1溶细胞素决定因子的组织结构。胞溶素前体(cylL（L)、cylL(S)、cylM和cylB）的表达需要四个开放阅读框。其中两个开放阅读框的推断产物CylL(L)和CylL(S)构成溶细胞素前体，其结构与翻译后修饰的细菌素（称为lantibiotic）相似。粪肠杆菌溶细胞素的决定因子和l抗生素的表达单位在组织结构上存在相似性，这表明粪肠杆菌溶细胞素代表了该类的一个新分支，并且是已知的第一个具有毒素活性的分支。
Pheromone-responsive conjugative plasmids are unique to the species Enterococcus faecalis. Many pheromone-responsive plasmids, including those frequently isolated from sites of infection, express a novel cytolysin that possesses both hemolytic and bacteriocin activities. Further, this cytolysin has been shown to be a toxin in several disease models. In the present study, nucleotide sequence determination, mutagenesis, and complementation analysis were used to determine the organization of the E. faecalis plasmid pAD1 cytolysin determinant. Four open reading frames are required for expression of the cytolysin precursor (cylL(L), cylL(S), cylM, and cylB). The inferred products of two of these open reading frames, CylL(L) and CylL(S), constitute the cytolysin precursor and bear structural resemblance to posttranslationally modified bacteriocins termed lantibiotics. Similarities between the organization of the E. faecalis cytolysin determinant and expression units for lantibiotics exist, indicating that the E. faecalis cytolysin represents a new branch of this class and is the first known to possess toxin activity.