Bioactive cyclic peptides from the psychrotolerant fungus Penicillium algidum
Bioactive cyclic peptides from the psychrotolerant fungus Penicillium algidum
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DOI：
10.1038/ja.2005.16
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发表时间：
2005-02-01
影响因子：
3.3
通讯作者：
Christophersen, C
中科院分区：
文献类型：
--
作者：
Dalsgaard, PW;Larsen, TO;Christophersen, C
利用C-18快速柱层析、LH-20葡聚糖凝胶和制备型HPLC从耐冷真菌Penicillium algidum中分离得到一个新的环状硝基肽--嗜冷素D（1），以及两个已知的环状肽--环肽A（2）和环肽D（3）。根据质谱信息、ID和2DNMR谱以及Marveland的方法，推测了嗜冷素D（1）的结构。在抗微生物、抗病毒、抗癌和抗疟原虫测定中测试化合物。在P388小鼠白血病细胞试验中，嗜冷蛋白D（1）显示出中等活性（ID 50 10.1 μ g/ml）。环孢素A（2）和D（3）对恶性疟原虫表现出中等活性（IC 50分别为3.5和4.7 μ g/ml）。
A new cyclic nitropeptide, psychrophilin D (1), together with two known cyclic peptides, cycloaspeptide A (2) and cycloaspeptide D (3), were isolated from the psychrotolerant fungus Penicillium algidum using C-18 flash chromatography, LH-20 Sephadex and preparative HPLC. The structure of psychrophilin D (1) was derived from mass spectrometric information, ID and 2D NMR spectra and Marfey's method. The compounds were tested in antimicrobial, antiviral, anticancer and antiplasmodial assays. Psychrophilin D (1) exhibited a moderate activity (ID50 10.1 mug/ml) in the P388 murine leukaemia cell assay. Cycloaspeptide A (2) and D (3) exhibited moderate activity (IC50 3.5 and 4.7 mug/ml, respectively) against Plasmodium falciparum.