RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC QUANTIFICATION OF VALEPOTRIATES IN HAIRY ROOT CULTURES OF VALERIANA-OFFICINALIS L VAR SAMBUCIFOLIA MIKAN
RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC QUANTIFICATION OF VALEPOTRIATES IN HAIRY ROOT CULTURES OF VALERIANA-OFFICINALIS L VAR SAMBUCIFOLIA MIKAN
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DOI：
10.1002/pca.2800050605
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发表时间：
1994-11-01
影响因子：
3.3
通讯作者：
VUAGNAT, P
中科院分区：
文献类型：
--
作者：
GRANICHER, F;CHRISTEN, P;VUAGNAT, P
缬草中缬草三酯的定量测定报道了一种新的高效液相色谱（HPLC）方法测定接骨木毛状根的方法。 定量基于在一个波长 (208 nm) 下同时等度测定单烯和二烯衍生物。 该分析快速且重复性高，得到的结果与当前使用的 HPLC 方法获得的结果相似，其中使用两次单独的运行进行戊三酸定量。
The quantitative determination of valepotriates in Valeriana officinalis L. var. sambucifolia Mikan hairy roots by a new high performance liquid chromatographic (HPLC) method is reported. The quantification is based on the simultaneous isocratic determination of the monoene and diene derivatives at one wavelength (208 nm). The analysis is rapid and highly reproducible, giving results similar to those obtained with a currently used HPLC method in which valepotriate quantification is undertaken using two separate runs.