Protective effects of fullerenol on carbon tetrachloride-induced acute hepatotoxicity and nephrotoxicity in rats
Protective effects of fullerenol on carbon tetrachloride-induced acute hepatotoxicity and nephrotoxicity in rats
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富勒醇对四氯化碳所致大鼠急性肝肾毒性的保护作用
DOI：
10.1016/j.carbon.2009.12.029
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发表时间：
2010-04-01
期刊：
影响因子：
10.9
通讯作者：
Li, Qing-Nuan
中科院分区：
文献类型：
--
作者：
Xu, Jing-Ying;Su, Yuan-Yuan;Li, Qing-Nuan
富勒烯醇的一个重要的生物学相关性质。本研究采用四氯化碳（CCl_4）诱导的大鼠肝、肾毒性模型，研究富勒烯醇对大鼠肝、肾损伤的保护作用及其可能机制（0、1、15和5 mg/kg/d），在CCl 4激发前3天、CCl 4激发后24 h，所有大鼠均采用血清和组织匀浆生物标志物以及病理学评价进行评估。所有结果表明，CCl 4引起血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性显著升高，以及血尿素氮和肌酐浓度丙二醛水平也显著升高，而组织匀浆中还原型谷胱甘肽与氧化型谷胱甘肽的比值降低。病理学评价表明，CCl 4 Fullerenol预处理显著减轻了生物标志物变化以及组织学变化，单独富勒烯醇预处理可以增加还原型谷胱甘肽与氧化型谷胱甘肽的比例，表明富勒烯醇可以通过提高抗氧化能力来保护组织免受CCl 4诱导的氧化应激（C）2010 Elsevier Ltd版权所有
An important biologically-relevant property of fullerenol. is its ability to quench free radicals Carbon tetrachloride (CCl4)-induced hepatotoxicity and nephrotoxicity model was used to investigate the possible mechanisms of fullerenol protection in Sprague-Dawley rats in this study Rats were administrated with fullerenol (0, 1, 15 and 5 mg/kg d) by intravenous or intraperitoneal injection for 3 days before CCl4 challenge, 24 h following the CCl4 challenge, all the rats were assessed using serum and tissue homogenates biomarkers as well as the pathological evaluation All results showed that CCl4 caused significant increasing serum activity of alanine aminotransferase and aspartate aminotransferase, as well as the concentration of blood urea nitrogen and creatinine Malondialdehyde level was also increased significantly, whereas the ratio of reduced glutathione to oxidized glutathione was decreased in tissue homogenates The pathological evaluation indicated the liver and kidney were damaged by CCl4 Fullerenol-pretreatment alleviated biomarker changes as well as histological changes significantly, and fullerenol-pretreatment alone can increase the ratio of reduced glutathione to oxidized glutathione, which indicated fullerenol could protect tissues against CCl4-induced oxidative stress by improving the antioxidant ability (C) 2010 Elsevier Ltd All rights reserved