Assessment of the local clonal spread of Streptococcus pneumoniae serotype 12F caused invasive pneumococcal diseases among children and adults
Assessment of the local clonal spread of Streptococcus pneumoniae serotype 12F caused invasive pneumococcal diseases among children and adults
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DOI：
10.1016/j.jiph.2019.05.019
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发表时间：
2019-11-01
影响因子：
6.7
通讯作者：
Nomoto, Ryohei
中科院分区：
文献类型：
--
作者：
Nakanishi, Noriko;Yonezawa, Takeshi;Nomoto, Ryohei
背景资料：我们对日本科比市侵袭性肺炎球菌病（IPD）肺炎链球菌血清型的分布进行了积极的监测，并对分离株之间的遗传相关性进行了研究。通过在日本兵库县科比市的主动监测，分析了2016年3月至2018年5月期间的肺炎菌株。血清型确定多重血清分型PCR和Quellung反应与肺炎球菌抗血清。结果：45株分离株中，12 F（n= 14，31%）、24 F（n=5，11%）和10A（n=4，9%）是最常见的血清型。对14株Sp12 F菌株进行多位点序列分型（MLST），将其分为ST 4846（n =4）和ST 6495（n =10）。WGS显示10株ST 6495的克隆性，基因组中只有13个单核苷酸多态性。同时，科比的ST 4846株与2018年日本鹤冈市报告的ST 4846暴发株仅存在差异。结论：血清型监测显示，科比市的ST 4846株以Sp12 F为主，WGS结果显示ST 6495在该市存在克隆传播。因此，Sp12 F的传播可能成为日本严重的公共卫生问题，未来需要进行全面监测。(C)2019年，任作家。由Elsevier Limited代表沙特国王本阿卜杜勒阿齐兹健康科学大学出版。这是一个在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Background: We conducted active surveillance to elucidate the distribution of Streptococcus pneumoniae serotypes causing invasive pneumococcal disease (IPD) and clarified the genetic relatedness among the isolates in Kobe City, Japan.Methods: Forty-five IPD-causing S. pneumoniae strains were analyzed from March 2016 to May 2018 through active surveillance in Kobe City, Hyogo, Japan. Serotypes were determined by multiplex serotyping PCR and the Quellung reaction with pneumococcal antisera. Fourteen Sp12F strains were subjected to whole-genome sequencing (WGS).Results: Among 45 isolates, the most frequent serotypes were 12F (n= 14, 31%), 24F (n=5, 11%), and 10A (n=4, 9%). Multilocus sequence typing (MLST) analysis of 14 isolates of Sp12F divided them into ST4846 (n =4) and ST6495 (n =10). WGS showed clonality of the 10 isolates of ST6495, with only 13 single nucleotide polymorphisms in the genomes. Meanwhile, ST4846 strains in Kobe differed from only the outbreak strains of Sp12F ST4846 in Tsuruoka, Japan, reported on 2018.Conclusions: Serotype monitoring showed Sp12F to be the predominant serotype in Kobe, and WGS revealed the clonal spread of Sp12F ST6495 in this city. Thus, the spread of Sp12F could become a serious public health problem in Japan, warranting thorough monitoring in future. (C) 2019 The Authors. Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).