Cation-dependent light-induced structural changes in visual receptor membranes.
Cation-dependent light-induced structural changes in visual receptor membranes.
复制标题
视觉受体膜中阳离子依赖性光诱导的结构变化。
DOI：
10.1016/0006-291x(73)91201-1
复制
发表时间：
1973
影响因子：
3.1
通讯作者：
Ian C.P. Smith
中科院分区：
文献类型：
--
作者：
Surendra P. Verma;Lawrence J. Berliner;Ian C.P. Smith
使用硬脂酰胺自旋探针研究钠离子和钙离子存在下杆外节膜中光诱导的结构变化。计算了在黑暗和光照下探针重定向的相关时间（τC）。在钠离子存在下，τC在黑暗中为3.3 × 10− 9秒，在光照下为2.7 × 10− 9秒;而在钙离子存在下，τC在黑暗中为2.9 × 10− 9秒，在光照下为3.6 × 10− 9秒。还计算了20°C下不同粘度的甘油水溶液中探针重定向的相关时间。τC值（暗和亮）的比较表明ROS中局部迁移率的变化对应于约150 cp的宏观粘度差。钙离子与带负电荷的基团的相互作用和形成的席夫碱的意义被强调。
A stearamide spin probe was used to study the light-induced structural changes in Rod Outer Segment Membranes in the presence of sodium and calcium ions. The correlation time (τC) for the reorientation of the probe was calculated in the dark and light. In the presence of sodium ions,τC= 3.3 × 10−9sec in the dark, and 2.7 × 10−9sec in the light while the opposite was noticed in the presence of calcium ions,τC= 2.9 × 10−9sec in the dark and 3.6 × 10−9sec in the light. The correlation times for reorientation of the probe were also calculated in aqueous glycerol solutions of varying viscosities at 20°C. Comparison of the values ofτC(dark and light) suggests a change in local mobility in the ROS corresponding to a macroscopic viscosity difference of approximately 150 cp. The significance of calcium ion interaction with negatively charged groups and the formation of a Schiff base is emphasized.