Epoxide hydrolase activity in the mitochondrial fraction of mouse liver
Epoxide hydrolase activity in the mitochondrial fraction of mouse liver
复制标题
小鼠肝脏线粒体部分中的环氧化物水解酶活性
作者：
S. Gill;B. Hammock
对环氧化异源生物的代谢命运的强烈兴趣是由于几个因素。例如，环氧化物通常是生物系统排泄烯烃和芳香族化合物所必需的亲脂体转化为亲脂体的中间体1，并且广泛存在于人类的天然和人造来源的饮食中。这些环氧化化合物中的一些可能会烷基化蛋白质和核酸，因此包括一些最有效的细胞毒素，诱变剂和致癌物质已知2。在哺乳动物中，环氧化物可以重排，脱附为烯烃，与谷胱甘肽反应形成缀合物，或在有或没有酶催化的情况下被水水解产生1，2-二醇1，3，4，。催化二醇形成的酶被称为环氧化物水解酶（EC 3.3.2.3），其亚细胞分布是本报告的主题。早期的数据表明，氧化苯乙烯水解酶活性与微粒体亚细胞组分有关5。环氧化物水解酶活性随后在核6，高尔基体和质膜7，并在细胞的胞质溶胶8，9，离开线粒体作为最后一个主要的细胞器被认为是缺乏环氧化物水解酶活性。我们现在报告了线粒体中大量环氧化物水解酶活性发生的有力证据。
The intense interest in the metabolic fate of epoxidized xeno-biotics is due to several factors. For instance, epoxides are often intermediates in the lipophile to hydrophile conversions necessary for the excretion of olefinic and aromatic compounds by living systems1, and are widely encountered in man's diet from both natural and man–made sources. Some of these epoxidized compounds may alkylate proteins and nucleic acids and thus include some of the most potent cytotoxins, mutagens and carcinogens known2. In mammals, epoxides may rearrange, deoxygenate to olefins, react with glutathione to form conjugates, or be hydrolysed by water to yield 1,2–diols with or without enzymatic catalysis1,3,4,. The enzymes which catalyse the formation of diols are known as epoxide hydrolases (EC 3.3.2.3), and their subcellular distribution is the subject of this report. Early data showed that styrene oxide hydrolase activity was associated with the microsomal subcellular fraction5. Epoxide hydrolase activity was subsequently demonstrated on the nuclear6, Golgi apparatus and plasma membranes7, and in the cytosol of the cell8,9, leaving the mitochondria as the last major cellular organelle assumed to be devoid of epoxide hydrolase activity. We now report strong evidence for the occurrence of substantial epoxide hydrolase activity in the mitochondria.