Refinement of the x-ray structure of the RC–LH1 core complex from Rhodopseudomonas palustris by single-molecule spectroscopy
Refinement of the x-ray structure of the RC–LH1 core complex from Rhodopseudomonas palustris by single-molecule spectroscopy
复制标题
通过单分子光谱精修沼泽红假单胞菌 RC-LH1 核心复合物的 X 射线结构
DOI：
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发表时间：
2007
影响因子：
11.1
通讯作者：
J. Köhler
中科院分区：
文献类型：
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作者：
M. Richter;J. Baier;J. Southall;R. Cogdell;S. Oellerich;J. Köhler
一个独特的单分子光谱与数值模拟相结合，使我们能够实现一个精细的结构模型的细菌叶绿素a（BChl a）的色素安排在反应中心捕光1核心复合物的红球藻沼泽。在光谱中的细节，如光谱分离和光谱带的相互偏振，进行了比较，从数值模拟的各种型号的BChl的安排，所有的可用的X射线结构的准确性在4.8- 100的限制内的结果。实验数据是一致的几何形状，其中15 BChl a二聚体，每个采取同源的捕光2复合物从嗜酸红球藻，被安排在一个整体的椭圆形结构，具有在椭圆的长边的间隙。
A unique combination of single-molecule spectroscopy with numerical simulations has allowed us to achieve a refined structural model for the bacteriochlorophyll a (BChl a) pigment arrangement in reaction center–light-harvesting 1 core complexes of Rhodopseudomonas palustris. Details in the optical spectra, such as spectral separation and mutual polarizations of spectral bands, are compared with results from numerical simulations for various models of the BChl a arrangement that were all well within the 4.8-Å limit of the accuracy of the available x-ray structure. The experimental data are consistent with a geometry where 15 BChl a dimers, each taken homologous to those from light-harvesting 2 complex from Rhodopseudomonas acidophila, are arranged in an overall elliptical structure featuring a gap on the long side of the ellipse.
影响因子：
5.7
作者：
Koepke, J;Hu, XC;Michel, H
通讯作者：
Michel, H