Imbalance between Th17 and regulatory T-cells in systemic lupus erythematosus
Imbalance between Th17 and regulatory T-cells in systemic lupus erythematosus
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DOI：
10.5603/fhc.2011.0088
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发表时间：
2011-01-01
影响因子：
1.5
通讯作者：
Musial, Jacek
中科院分区：
文献类型：
--
作者：
Kleczynska, Weronika;Jakiela, Bogdan;Musial, Jacek
调节性T细胞（Treg）功能受损导致免疫耐受失败并引发自身免疫。我们分析了系统性红斑狼疮（SLE）中Treg的缺乏是否伴随着效应T细胞应答的增加。我们通过流式细胞术检测PMA/离子霉素刺激的7例活动期SLE患者、8例缓解期SLE患者和11例健康对照者的血淋巴细胞中产生IL-17 A（Th 17）和IFN γ（Th 1）的CD 4（+）T细胞的频率。将循环Treg评价为表达FoxP 3的CD 4（+）CD 25（+）淋巴细胞。两组之间Treg细胞的百分比没有差异，但与非活动性SLE（11 [6-15]; p = 0.05）和健康对照（16[10-20]; p < 0.01）相比，活动性SLE（5 [1-7]个细胞/μ L）中Treg细胞的绝对计数降低。与对照组相比，SLE患者Th 1细胞的频率和数量均降低。在Th 17细胞的数量上没有观察到差异，这导致Th 1/Th 17比率降低。同时，观察到与活动性SLE（1.1 [1.0-2.1]; p < 0.05）相比，健康对照（2.2 [1.8-3.6]）中的Treg/Th 17比率更高。Treg细胞数量与疾病活动状态（SLEDAI，r = -0.59）相关。处于疾病活动期的SLE患者的特征是Treg细胞缺乏和Treg/Th 17比例降低。这表明，主要T细胞亚群之间的失衡可能是负责增加促炎反应在SLE的恶化。（Folia Histochemica et Cytobiologica 2011; Vol.49，No.4，pp.（第646-653段）
Impaired function of regulatory T-cells (Treg) leads to a failure in immune tolerance and triggers autoimmunity. We analyzed whether the deficiency in Treg in systemic lupus erythematosus (SLE) is accompanied by an increase in effector T-cell responses. We studied the frequencies of IL-17A (Th17) and IFN gamma (Th1) producing CD4(+) T-cells by flow cytometric detection of intracellular cytokines in PMA/ionomycin stimulated blood lymphocytes from seven patients with active SLE, eight with SLE in remission, and 11 healthy controls. Circulating Treg were evaluated as CD4(+)CD25(+) lymphocytes expressing FoxP3. There was no difference in the percentage of Treg cells between the groups, but their absolute counts were decreased in active SLE (5 [1-7] cells/mu L) compared to inactive SLE (11 [6-15]; p = 0.05) and healthy controls (16[10-20]; p < 0.01). Both the frequency and numbers of Th1 cells were decreased in SLE compared to controls. No difference was observed in the number of Th17 cells, which resulted in a decreased Th1/Th17 ratio. In parallel, a higher Treg/Th17 ratio in healthy controls (2.2 [1.8-3.6]) compared to active SLE (1.1 [1.0-2.1]; p < 0.05) was observed. There was a correlation between the number of Treg cells and disease activity status (SLEDAI, r = -0.59). SLE patients in the active phase of the disease are characterized by a deficiency in Treg cells and decreased Treg/Th17 ratio. This suggests that the imbalance between major T-cells subsets might be responsible for an increased proinflammatory response in the exacerbation of SLE. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 646-653)