A subcloning strategy for DNA sequence analysis.
A subcloning strategy for DNA sequence analysis.
复制标题
DNA 序列分析的亚克隆策略。
DOI：
10.1093/nar/8.23.5541
复制
发表时间：
1980
影响因子：
14.9
通讯作者：
H. Lehrach
中科院分区：
文献类型：
--
作者：
A. Frischauf;H. Garoff;H. Lehrach
我们在这里描述了一种新的片段制备策略，用于使用末端标记的DNA片段作为底物的测序程序（2，3），该策略可直接应用于克隆到pBR 322的Pst I位点的DNA片段，或以修饰形式插入到相同质粒的BamHI或Sal I位点的DNA片段。生成预定重叠的亚克隆的有序集合。这些可以直接测序而无需进一步的链或片段分离步骤。
We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced directly without further strand- or fragment separation steps.