Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor
Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor
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DOI：
10.1016/j.ijhydene.2008.05.033
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发表时间：
2008-10
影响因子：
7.2
通讯作者：
Wanqian Guo;N. Ren;Xiangjing Wang;W. Xiang;Zhao-Hui Meng;Jie Ding;Y. Qu;Luyan Zhang
中科院分区：
文献类型：
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作者：
Wanqian Guo;N. Ren;Xiangjing Wang;W. Xiang;Zhao-Hui Meng;Jie Ding;Y. Qu;Luyan Zhang
采用颗粒活性炭（GAC）强化膨胀颗粒污泥床（EGSB）工艺，利用糖蜜废水进行混合微生物发酵产氢。在整个运行期间未进行pH调节。EGSB反应器在35°C下运行时，产氢速率（HPR）最大，为0.71L/Lh，具有较高的产氢能力。产氢率（HY）最高为3.47mol/mol蔗糖，最大比产氢速率（SHPR）为3.16mmol H2/g VSSh。氢气体积含量估计为总生物气的30-53%，并且在整个研究期间生物气不含甲烷。溶解的发酵产物主要是乙酸和乙醇，少量的丙酸，丁酸和戊酸。发现高氢气产率总是与高水平的乙醇生产相关联。当pH值和碱度分别为4.2- 4.4mgCaCO_3/L和280- 340 mgCaCO_3/L时，形成稳定的乙醇型发酵，乙醇和乙酸的浓度之和占总液体产物的89.1%。平均附着生物膜浓度估计为17.1g/L，这有利于高效产氢。EGSB系统在高有机负荷下具有较高的生物量截留率，是一种很有前途的高浓度废水生物制氢工艺。
An expanded granular sludge bed (EGSB) process with granular activated carbon (GAC) was developed for fermentative hydrogen production from molasses-containing wastewater by mixed microbial cultures. No pH regulation was performed during the whole operation period. Running at the temperature of 35°C, the EGSB reactor presented a high hydrogen production ability as the hydrogen production rate (HPR) maximized at 0.71L/Lh. At the same time, the hydrogen yield (HY) peaked at 3.47mol/mol sucrose and the maximum specific hydrogen production rate (SHPR) was found to be 3.16mmol H2/g VSSh. Hydrogen volume content was estimated to be 30–53% of the total biogas and the biogas was free of methane throughout the study. Dissolved fermentation products were predominated by acetate and ethanol, with smaller quantities of propionate, butyrate and valerate. It was found that high hydrogen yield was always associated with a high level of ethanol production. When the pH value and alkalinity ranged from 4.2–4.4mg CaCO3/L to 280–340mg CaCO3/L, respectively, stable ethanol-type fermentation was formed with the sum of ethanol and acetate concentration ratio of 89.1% to the total liquid products. The average attached biofilm concentration was estimated to be 17.1g/L, which favored hydrogen production efficiently. With high biomass retention at high organic loading rate (OLR), this EGSB system showed to be a promising high-efficient bioprocess for hydrogen production from high-strength wastewater.