Biophysical stimuli on cells during tissue differentiation at implant interfaces
Biophysical stimuli on cells during tissue differentiation at implant interfaces
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DOI：
10.1016/s0021-9290(96)00140-6
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发表时间：
1997-06-01
影响因子：
2.4
通讯作者：
Soballe, K
中科院分区：
文献类型：
--
作者：
Prendergast, PJ;Huiskes, R;Soballe, K
如果肌肉骨骼组织的机械功能确实有效，最合理的假设是，这是因为组织中的机械环境影响细胞的分化和表达。虽然机械刺激可以影响生物活性因子的运输、细胞变形和细胞骨架应变，但它们是否有可能调节组织分化序列(例如，在骨折愈合或胚胎发生期间)的问题尚未得到回答。为了评估生物物理刺激作为组织分化介质的可行性，我们分析了植入犬关节突内的微动装置附近的界面组织形成。使用两相有限元模型，并根据(I)与植入物运动相反的力、(Ii)成分之间的相对速度、(Iii)流体压力、(Iv)组织变形和(V)组织中的应变来表征组织中的力学环境。据预测，随着组织分化的进行，界面组织中的细胞会发生细微但系统性的机械变化。具体地说，随着与运动相反的力的增加，种植体从由最大允许位移控制(运动控制)转变为由最大可用载荷控制(力控制)。这会导致流体相相对于固相的速度降低，并导致间质流体压力下降，同时假体周围组织应变减少。生物物理刺激在组织内的变化可以被描绘为“机械调节通路”，它将从运动控制到力控制的转变识别为组织分化序列中的一个分支事件。(C)1997年爱思唯尔科学有限公司。
If musculoskeletal tissues are indeed efficient for their mechanical function, it is most reasonable to assume that this is achieved because the mechanical environment in the tissue influences cell differentiation and expression. Although mechanical stimuli can influence the transport of bioactive factors, cell deformation and cytoskeletal strain, the question of whether or not they have the potential to regulate tissue differentiation sequences (for example, during fracture healing or embryogenesis) has not been answered.To assess the feasibility of biophysical stimuli as mediators of tissue differentiation, we analysed interfacial tissue formation adjacent to a micromotion device implanted into the condyles of dogs. A biphasic finite element model was used and the mechanical environment in the tissue was characterised in terms of (i) forces opposing implant motion, (ii) relative velocity between constituents, (iii) fluid pressure, (iv) deformation of the tissue and (v) strain in the tissue. It was predicted that, as tissue differentiation progressed, subtle but systematic mechanical changes occur on cells in the interfacial tissue. Specifically, as the forces opposing motion increase, the implant changes from being controlled by the maximum-allowable displacement (motion-control) to being controlled by the maximum-available load (force-control). This causes a decrease in the velocity of the fluid phase relative to the solid phase and a drop in interstitial fluid pressure accompanied by a reduction in peri-prosthetic tissue strains. The variation of biophysical stimuli within the tissue can be plotted as 'mechano-regulatory pathway', which identifies the transition from motion-control to force-control as a branching event in the tissue differentiation sequence. (C) 1997 Elsevier Science Ltd.