Expression of transforming growth factor beta 1 by human endometrial carcinoma cell lines: inverse correlation with effects on growth rate and morphology.
Expression of transforming growth factor beta 1 by human endometrial carcinoma cell lines: inverse correlation with effects on growth rate and morphology.
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发表时间：
1990-06
期刊：
影响因子：
11.2
通讯作者：
J. Boyd;D. Kaufman
中科院分区：
文献类型：
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作者：
J. Boyd;D. Kaufman
我们研究了转化生长因子β 1（TGF-β 1）在体外对人子宫内膜癌（HEC）细胞系细胞生物学各个方面的影响，以及这些细胞系TGF-β 1 mRNA的表达。研究了来自8个HEC肿瘤的细胞系，代表了各种组织学亚型，以测试关于TGF-β 1对这种特定肿瘤细胞类型的影响的结论的一般性。TGF-β 1（10 ng/ml）可抑制5种HEC细胞系的生长，而3种细胞系的生长未受到抑制。对生长的影响与TGF-β 1诱导的形态学改变相关;生长受抑制的细胞系显示出更大、更平坦、更接触抑制的表型，而生长未受抑制的细胞系对TGF-β 1的反应几乎没有明显的形态学改变。对TGF-β 1 mRNA水平的北方分析显示，对TGF-β 1处理无反应的三种HEC细胞系表达相对大量的TGF-β 1。相应地，对TGF-β 1产生生长和形态学变化的5种HEC细胞系表达的TGF-β 1 mRNA水平低得多。这些结果表明，人HEC细胞系对TGF-β 1的敏感性是可变的，并且这种敏感性与TGF-β 1的表达水平呈负相关。
We examined the effects of transforming growth factor beta 1 (TGF-beta 1) on various aspects of the cell biology of human endometrial carcinoma (HEC) cell lines in vitro, as well as the expression of TGF-beta 1 mRNA by these cell lines. Cell lines from eight HEC tumors, representing a variety of histological subtypes, were studied in order to test the generality of conclusions regarding the effects of TGF-beta 1 on this particular tumor cell type. The growth of five HEC cell lines was inhibited by TGF-beta 1 (10 ng/ml), while growth of three cell lines was not inhibited. The effects on growth correlated with morphological alterations induced by TGF-beta 1; the cell lines with inhibited growth displayed a larger, flatter, more contact-inhibited phenotype, while the cell lines whose growth ws not inhibited showed few discernible morphological alterations in response to TGF-beta 1. Northern analysis of TGF-beta 1 mRNA levels revealed that the three HEC cell lines unresponsive to TGF-beta 1 treatment expressed relatively large amounts of TGF-beta 1. Correspondingly, the five HEC cell lines which responded to TGF-beta 1 with growth and morphological changes expressed much lower levels of TGF-beta 1 mRNA. These results suggest that the sensitivity of human HEC cell lines to TGF-beta 1 is variable and that this sensitivity is inversely correlated with the level of expression of TGF-beta 1.