Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes
Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes
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DOI：
10.1086/315653
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发表时间：
2000-07-01
影响因子：
6.4
通讯作者：
Arntzen, CJ
中科院分区：
文献类型：
--
作者：
Tacket, CO;Mason, HS;Arntzen, CJ
一种提供疫苗抗原的新方法是使用廉价、充足的植物性口服疫苗，将诺沃克病毒衣壳蛋白（NVCP）组装成病毒样颗粒，用作测试抗原，以确定摄入转基因马铃薯的志愿者是否能产生免疫反应。 24 名健康成年志愿者接受 2 或 3 剂转基因马铃薯（n = 20）或 3 剂野生型马铃薯（n = 4），每剂由 150 克生的、去皮的切丁马铃薯组成，其中含有 215-751 微克 NVCP。摄入转基因马铃薯的 20 名志愿者中，有 19 名 (95%) 的特异性 IgA 抗体分泌细胞数量显着增加，20 名志愿者中的 4 名 (20%) 出现了特异性血清 IgG，20 名志愿者中的 6 名 (30%) 出现了特异性粪便 IgA。总体而言，20 名志愿者中有 19 名出现了某种免疫反应，尽管血清抗体水平增加幅度不大。
A new approach for delivering vaccine antigens is the use of inexpensive, plentiful, plant-based oral vaccines, Norwalk virus capsid protein (NVCP), assembled into virus-like particles, was used as a test antigen, to determine whether immune responses could be generated in volunteers who ingested transgenic potatoes. Twenty-four healthy adult volunteers received 2 or 3 doses of transgenic potato (n = 20) or 3 doses of wild-type potato (n = 4), Each dose consisted of 150 g of raw, peeled, diced potato that contained 215-751 mu g of NVCP. Nineteen (95%) of 20 volunteers who ingested transgenic potatoes developed significant increases in the numbers of specific IgA antibody-secreting cells, Four (20%) of 20 volunteers developed specific serum IgG, and 6 (30%) of 20 volunteers developed specific stool IgA. Overall, 19 of 20 volunteers developed an immune response of some kind, although the level of serum antibody increases was modest.