Induction of myocardial biglycan in heart failure in rats -: an extracellular matrix component targeted by AT1 receptor antagonism
Induction of myocardial biglycan in heart failure in rats -: an extracellular matrix component targeted by AT1 receptor antagonism
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DOI：
10.1016/j.cardiores.2003.08.017
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发表时间：
2003-12-01
影响因子：
10.8
通讯作者：
Attramadal, H
中科院分区：
文献类型：
--
作者：
Ahmed, MS;Oie, E;Attramadal, H
目的：与充血性心力衰竭相关的心脏重塑通常涉及心室腔扩张、心肌细胞肥大和细胞外基质改变。双糖蛋白聚糖是一种细胞外蛋白聚糖，具有几种最近被认识到的功能，包括细胞粘附、胶原原纤维组装和生长因子相互作用。本研究旨在探讨心力衰竭（HF）模型心肌组织中双糖链蛋白聚糖（biglycan）的表达调控及合成位点。方法：结扎大鼠左冠状动脉，建立心肌梗死模型。采用北方杂交和实时荧光定量RT-PCR检测mRNA水平。用原位杂交和免疫组织化学方法分析双糖链蛋白聚糖的细胞分布。结果如下：与假手术大鼠相比，心力衰竭大鼠左心室和右心室的非缺血组织中的心肌双糖蛋白聚糖mRNA水平显著升高。尽管表达水平在MI后7天达到峰值（与假手术组相比增加13倍，P
Objective: Cardiac remodelling associated with congestive heart failure typically involves dilatation of the ventricular cavities, cardiomyocyte hypertrophy and alterations of extracellular matrix. Biglycan is an extracellular proteoglycan with several recently appreciated functions including cell adhesion, collagen fibril assembly, and growth factor interactions. The aims of this study were to investigate the regulation of biglycan expression and to elucidate the site(s) of synthesis of biglycan in myocardial tissue in an experimental model of heart failure (HF). Methods: Myocardial tissue samples were obtained from rats with myocardial infarction (MI) subsequent to ligation of the left coronary artery. Northern blot analysis and real-time quantitative RT-PCR were employed to investigate mRNA levels. The cellular distribution of biglycan was analysed by in situ hybridisation and immunohistochemistry. Results: Myocardial biglycan mRNA levels in non-ischemic tissue of both left and right ventricles of heart failure rats were substantially elevated as compared to sham-operated rats. Although expression levels peaked 7 days after MI (13-fold increase compared to the sham group, P