Viability, morphology and proteome of M. smegmatis MSMEG_0319 knockout strain
Viability, morphology and proteome of M. smegmatis MSMEG_0319 knockout strain
复制标题
耻垢分枝杆菌 MSMEG_0319 敲除菌株的活力、形态和蛋白质组
DOI：
10.1002/pmic.201500054
复制
发表时间：
2016
期刊：
影响因子：
3.4
通讯作者：
Yufang Ma
中科院分区：
文献类型：
--
作者：
Shanshan Sha;Xiaoxia Shi;Liming Xu;Jiabin Wen;Yi Xin;Yufang Ma
Mycobacterium tuberculosisRv0228, a membrane protein, is predicted as a drug target through computational methods. MSMEG_0319 (MS0319) inMycobacterium smegmatismc2155 is the ortholog of Rv0228. To study the effect of MS0319 protein onM. smegmatis, anMS0319gene knockout strain (ΔMS0319) was generated via a homologous recombination technique in this study. The results showed that the lack of MS0319 protein in mc2155 cells led to the loss of viability at nonpermissive temperature. Scanning electron microscopy and transmission electron microscopy observations showed drastic changes in cellular shape especially cell wall disruption in ΔMS0319 cells. Proteomic analysis of ΔMS0319 cells through LC‐MS/MS revealed that 462 proteins had changes in their expressions by lacking MS0319 protein. TheM. tuberculosisorthologs of these 462 proteins were found through BLASTp search and functional clustering and metabolic pathway enrichment were performed on the orthologs. The results revealed that most of them were enzymes involved in metabolism of carbohydrates and amino acids, indicating that Rv0228 played an important role in cellular metabolism. All these results suggested Rv0228 as a potential target for development of antituberculosis drugs.
