Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response
Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response
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DOI：
10.1023/a:1020780022549
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发表时间：
2003-01-01
影响因子：
5.1
通讯作者：
Chen, ZX
中科院分区：
文献类型：
--
作者：
Dong, JX;Chen, CH;Chen, ZX
WRKY蛋白是最近发现的一类dna结合蛋白，其识别大量植物防御相关基因启动子中的TTGAC(C/T) W-box元件。以含有W-box序列的低聚分子为探针，我们检测了水杨酸（SA）诱导的拟南芥WRKY dna结合活性。对拟南芥基因组的搜索发现了72个编码WRKY dna结合转录因子特征蛋白的基因，根据WRKY锌指基序的数量和结构可将其分为三组。Northern blotting分析显示，72个AtWRKY基因中有49个在感染了无毒菌株丁香假单胞菌或SA处理过的植物中存在差异调控。这些病原体和/或sa调控的WRKY基因可以根据其在野生型植物和防御信号通路缺陷突变体中的表达模式进一步分类。对预测翻译起始位点上游的5个序列的检查显示，在病原体和/或sa调控的拟南芥WRKY基因启动子中W盒大量富集。这些结果表明，WRKY基因的防御调控表达涉及转录因子超家族成员广泛的转录激活和抑制。
WRKY proteins are a recently identified class of DNA-binding proteins that recognize the TTGAC(C/T) W-box elements found in the promoters of a large number of plant defense-related genes. With oligo molecules containing the W-box sequences as probes, we detected a number of WRKY DNA-binding activities in Arabidopsis that were induced by salicylic acid (SA). Search of the Arabidopsis genome identifies 72 genes encoding proteins characteristic of WRKY DNA-binding transcription factors that can be divided into three groups based on the number and structures of their WRKY zinc-finger motifs. Northern blotting analysis revealed that 49 of the 72 AtWRKY genes were differentially regulated in the plants infected by an avirulent strain of the bacterial pathogen Pseudomonas syringae or treated by SA. These pathogen- and/or SA-regulated WRKY genes can be further categorized into groups based on their expression patterns in both wild-type plants and mutants defective in defense signaling pathways. Inspection of the 5 sequences upstream of the predicated translation start sites revealed a substantial enrichment of W boxes in the promoters of pathogen- and/or SA-regulated Arabidopsis WRKY genes. These results suggest that defense-regulated expression of WRKY genes involves extensive transcriptional activation and repression by its own members of the transcription factor superfamily.