Upregulated IL-23 and IL-17 in Behcet patients with active uveitis
Upregulated IL-23 and IL-17 in Behcet patients with active uveitis
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患有活动性葡萄膜炎的 Behcet 患者 IL-23 和 IL-17 上调
DOI：
10.1167/iovs.07-1390
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发表时间：
2008-07-01
影响因子：
4.4
通讯作者：
Kijlstra, Aize
中科院分区：
文献类型：
--
作者：
Chi, Wei;Zhu, Xuefei;Kijlstra, Aize
目的.白塞病（BD）是一种全身性炎症性疾病，可能由自身免疫反应引起。白细胞介素（IL）-23/IL-17通路已被证明参与某些炎性疾病的发展和维持。本研究旨在探讨IL-23和IL-17在BD中的作用。RT-PCR检测外周血单个核细胞（PBMCs）IL-23 p19 mRNA的表达。ELISA法检测血清和PBMC中IL-23、IL-17和IFN-γ水平。采用流式细胞术检测IL-17和IFN-γ产生的T细胞比例及CD 45 RO的表达。结果显示，在伴有活动性葡萄膜炎的BD患者中，IL-23 p19 mRNA、IL-23、IL-17和IFN-γ的表达显著升高。在患有活动性葡萄膜炎的BD患者中，来自PBMC的产生IL-17和产生IFN-γ的T细胞的频率显著上调。活动性葡萄膜炎患者IL-17水平升高（3.10% ± 0.53%）主要由CD 45 RO（+）记忆T细胞产生。重组（r）IL-23可上调多克隆刺激的PBMC产生IL-17，而干扰素（IFN）-γ可下调IL-17的产生。这些发现揭示了在患有活动性葡萄膜炎的BD患者中IL-23、IL-17和IFN-γ的水平升高，并且它们表明IL-23/ IL-17途径与IFN-γ一起与BD患者中的活动性眼内炎症相关。
PURPOSE. Behcet disease (BD) is a systemic inflammatory disease presumably caused by an autoimmune response. The interleukin (IL)-23/IL-17 pathway has been demonstrated to be involved in the development and maintenance of certain inflammatory diseases. This study was designed to investigate the role of IL-23 and IL-17 in BD.METHODS. IL-23p19 mRNA in peripheral blood mononuclear cells (PBMCs) was examined using RT-PCR. The levels of IL-23, IL-17, and IFN-gamma in sera or PBMCs were detected by ELISA. Flow cytometry was used to evaluate the frequencies of IL-17 producing and IFN-gamma-producing T cells and the expression of CD45RO.RESULTS. Results showed that the expression of IL-23p19 mRNA, IL-23, IL-17, and IFN-gamma was markedly elevated in BD patients with active uveitis. The frequencies of IL-17-producing and IFN-gamma-producing T cells from PBMCs were significantly upregulated in BD patients with active uveitis. The increased IL-17 (3.10% +/- 0.53%) in BD patients with active uveitis was primarily produced by CD45RO(+) memory T cells. Recombinant (r) IL-23 could upregulate IL-17 production by polyclonally stimulated PBMCs, whereas interferon (IFN)-gamma down-regulated IL-17 production.CONCLUSIONS. These findings reveal that the levels of IL-23, IL-17, and IFN-gamma are elevated in BD patients with active uveitis, and they suggest that the IL-23/ IL-17 pathway together with IFN-gamma is associated with the active intraocular inflammation in BD patients.