Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia.
Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia.
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DOI：
10.1016/j.jiph.2010.10.002
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发表时间：
2011-03-01
影响因子：
6.7
通讯作者：
Kebede, Nigatu
中科院分区：
文献类型：
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作者：
Gebretsadik, Simon;Kassa, Tesfu;Kebede, Nigatu
李斯特菌病是一种人畜共患疾病，是世界范围内重要的新发细菌性人畜共患疾病之一。在李斯特菌属的不同种中，已知单核增生李斯特菌（L. monocytogenes）可引起人类和动物的李斯特菌病，发病率低，但病死率高。在埃塞俄比亚的兽医和公共卫生部门，关于单核增生李斯特菌和其他李斯特菌种类的发生和分布的信息非常有限。本研究的目的是从埃塞俄比亚亚的斯亚贝巴的动物源性食品（松软干酪、生牛肉、生牛奶和液态全蛋）中分离和鉴定单核细胞增生乳杆菌和其他李斯特菌。2008年11月至2009年3月，采用横断面研究设计，随机采集动物源性食品样本391份。单增李斯特菌的分离和鉴定主要按照美国食品和药物管理局的程序进行。在检查的样本中，发现102个（26.1%）李斯特菌呈阳性。在生牛肉、液态全蛋、生牛奶和松软干酪样品中分离到李斯特菌分别为39（51.3%）、37（32.2%）、22（22%）和4（4%）种。在5.4%的样品中检出单增李斯特菌。主要来源于生牛奶（13%）和液态全蛋（4.3%），其次是生牛肉（2.6%）和白干酪（1%）。除单核增生李斯特菌外，其他李斯特菌种分别为innocua李斯特菌（60.8%）、L. welshimeri李斯特菌（6.9%）、L. seeligeri李斯特菌（3.9%）、L. murrayi李斯特菌（2.9%）、L. grayi李斯特菌（2.9%）和L. ivanovii李斯特菌（1.9%）。结果表明，在埃塞俄比亚首都亚的斯亚贝巴，单核增生乳杆菌和其他李斯特菌种类广泛分布于动物源性食品中。
Listeriosis is a disease of humans and animals, in which it is one of the important emerging bacterial zoonotic diseases worldwide. Among the different species of the genus Listeria, Listeria monocytogenes (L. monocytogenes) is known to cause listeriosis in humans and animals with low incidence but high case fatality rate. Information on the occurrence and distribution of L. monocytogenes and other Listeria species is very limited both in the veterinary and public health sectors in Ethiopia. The objective of this study was to isolate and characterize L. monocytogenes and other Listeria species from foods of animal origin (cottage cheese, raw beef, raw milk and liquid whole egg) in Addis Ababa, Ethiopia. A total of 391 food samples of animal origin were collected randomly, using a cross-sectional study design from November 2008 to March 2009. L. monocytogenes isolation and characterization were performed according to mainly the United States Food and Drug Administration procedures. Of the samples examined, 102 (26.1%) were found to be positive for Listeria. Listeria species were isolated in 39 (51.3%), 37 (32.2%), 22 (22%) and 4 (4%) of the raw beef, liquid whole egg, raw milk and cottage cheese samples respectively. L. monocytogenes was detected in 5.4% of the samples analyzed. It was isolated mainly from raw milk (13%) and liquid whole egg (4.3%) followed by raw beef (2.6%) and cottage cheese (1%). In addition to L. monocytogenes, other Listeria species were identified as L. innocua (60.8%), L. welshimeri (6.9%), L. seeligeri (3.9%), L. murrayi (2.9%) and L. grayi (2.9%) and L. ivanovii (1.9%). It was shown that L. monocytogenes and other Listeria species are widely spread in occurrence in foods of animal origin in Addis Ababa, Ethiopia.