Implications of Differential Stress Response Activation Following Non-Frozen Hepatocellular Storage
Implications of Differential Stress Response Activation Following Non-Frozen Hepatocellular Storage
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DOI：
10.1089/bio.2012.0045
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发表时间：
2013-02-01
影响因子：
1.6
通讯作者：
Van Buskirk, Robert G.
中科院分区：
文献类型：
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作者：
Corwin, William L.;Baust, John M.;Van Buskirk, Robert G.
肝细胞是许多细胞治疗和体外研究的关键。在这些应用中使用它们的一个限制因素是在不损失生存能力或功能的情况下处理和保存它们的能力。正常大鼠肝细胞(NHEP)和人肝癌(C3A)细胞被保存在4℃或37℃下，以检查后处理应激反应。白藜芦醇和沙鲁布林在储存过程中被用来确定靶向分子应激途径调节如何影响细胞存活。这项研究表明，储存结果取决于许多因素，包括：细胞类型、储存介质、储存长度、储存温度和化学调节剂。这些数据暗示了一种基于分子的应激反应，这种反应不是普遍的，但对细胞储存的条件集是特定的。此外，这些发现暗示了从诊断细胞选择到基于细胞的治疗，有针对性地保护或破坏特定细胞类型的许多应用的可能性。最终，这项研究证明了进一步深入分子研究生物处理和保存对细胞应激反应的必要性。
Hepatocytes are critical for numerous cell therapies and in vitro investigations. A limiting factor for their use in these applications is the ability to process and preserve them without loss of viability or functionality. Normal rat hepatocytes (NHEPs) and human hepatoma (C3A) cells were stored at either 4 degrees C or 37 degrees C to examine post-processing stress responses. Resveratrol and salubrinal were used during storage to determine how targeted molecular stress pathway modulation would affect cell survival. This study revealed that storage outcome is dependent upon numerous factors including: cell type, storage media, storage length, storage temperature, and chemical modulator. These data implicate a molecular-based stress response that is not universal but is specific to the set of conditions under which cells are stored. Further, these findings allude to the potential for targeted protection or destruction of particular cell types for numerous applications, from diagnostic cell selection to cell-based therapy. Ultimately, this study demonstrates the need for further in-depth molecular investigations into the cellular stress response to bioprocessing and preservation.